Tao Erxing, Song Qimin, Tang Jialin, Xin Wenqiang, Xiao Zhipeng, Liu Zhixin, Xie Guangbin
Jiangxi Key Laboratory of Neurological Diseases, Department of Neurosurgery, The First Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi, China.
Queen Mary School, Nanchang University, Nanchang, 330006, China.
Sci Rep. 2025 Apr 30;15(1):15240. doi: 10.1038/s41598-025-99499-3.
Glioblastoma (GBM) is the most common and deadly malignant tumor of the nervous system. RIMS1, a member of the RAS gene superfamily, plays a critical role in signaling pathways regulating cell growth and differentiation. However, the prognostic value of RIMS1, particularly its relationship with immune cell infiltration in gliomas, has not been fully explored. RIMS1 expression in glioblastoma cells and tissues was assessed using bioinformatics platforms. The association between RIMS1 expression levels and overall survival was analyzed using Kaplan-Meier analysis and Cox regression model. To evaluate the proliferative and migratory capacity of GBM cells, we conducted CCK-8, colony formation, transwell, and scratch assays. With data from The Cancer Genome Atlas (TCGA), we investigated the correlation between RIMS1 expression and immune cell infiltration levels and assessed the prognostic impact of RIMS1 on GBM patient survival, focusing on its potential involvement in immune pathways. Lower RIMS1 expression was associated with poorer overall survival and was linked to patient age, gender, and tumor grade. Importantly, RIMS1 expression showed a significant correlation with immune cell infiltration levels, suggesting that RIMS1 influences glioblastoma survival, at least in part, through immune-related mechanisms. In glioblastoma patients, elevated RIMS1 expression may serve as an independent prognostic biomarker, potentially through its impact on immune cell infiltration.
胶质母细胞瘤（GBM）是最常见且致命的神经系统恶性肿瘤。RIMS1是RAS基因超家族的成员之一，在调节细胞生长和分化的信号通路中起关键作用。然而，RIMS1的预后价值，尤其是其与胶质瘤中免疫细胞浸润的关系，尚未得到充分研究。利用生物信息学平台评估了胶质母细胞瘤细胞和组织中RIMS1的表达情况。使用Kaplan-Meier分析和Cox回归模型分析了RIMS1表达水平与总生存期之间的关联。为了评估GBM细胞的增殖和迁移能力，我们进行了CCK-8、集落形成、Transwell和划痕试验。利用来自癌症基因组图谱（TCGA）的数据，我们研究了RIMS1表达与免疫细胞浸润水平之间的相关性，并评估了RIMS1对GBM患者生存的预后影响，重点关注其在免疫途径中的潜在作用。RIMS1表达降低与较差的总生存期相关，并且与患者年龄、性别和肿瘤分级有关。重要的是，RIMS1表达与免疫细胞浸润水平显示出显著相关性，这表明RIMS1至少部分地通过免疫相关机制影响胶质母细胞瘤的生存。在胶质母细胞瘤患者中，RIMS1表达升高可能作为一种独立的预后生物标志物，可能是通过其对免疫细胞浸润的影响。
