Nakamura S, Gotoh S, Takenaka O, Takahashi K
J Biochem. 1985 Jun;97(6):1603-9. doi: 10.1093/oxfordjournals.jbchem.a135217.
Monkey (Macaca fuscata) mononuclear leukocytes were stimulated to produce thromboplastin (tissue factor) upon exposure to lipopolysaccharide, LPS. The stimulation was dose-related in the concentration range of 10(-5) to 10(-1) micrograms/ml of LPS. Lipid A portion of the LPS molecule was essential to induce the leukocyte ability for tissue factor generation. Thus, a lipid-lipid interaction between LPS and the cells is a plausible trigger for eliciting the ability. Approximately 50% of the tissue factor thus produced appeared to be located on the cell surface, at which the coagulation cascade is probably initiated via the activation of factor VII. Among monkey mononuclear cell populations, monocytes were responsible for LPS-induced tissue factor production. Lymphocytes amplified the basal ability of monocytes to produce the factor by two-fold at physiological lymphocyte-monocyte ratios of 8:1 to 10:1. This indicates a complementary effect of lymphocytes upon the LPS-mediated monocyte ability. The medium supernatant from LPS-stimulated lymphocytes affected the monocyte competence while the stimulated lymphocytes did not. This result suggests that a soluble product of lymphocytes, i.e., lymphokine-like mediator, but not the cellular entity, participates in LPS-induced tissue factor production of monocytes.
猕猴（日本猕猴）单核白细胞在接触脂多糖（LPS）后被刺激产生凝血活酶（组织因子）。在10^(-5)至10^(-1)微克/毫升的LPS浓度范围内，这种刺激呈剂量相关。LPS分子的脂质A部分对于诱导白细胞产生组织因子的能力至关重要。因此，LPS与细胞之间的脂质-脂质相互作用是引发该能力的一个合理触发因素。如此产生的约50%的组织因子似乎位于细胞表面，凝血级联反应可能在此通过激活因子VII而启动。在猕猴单核细胞群体中，单核细胞负责LPS诱导的组织因子产生。在生理淋巴细胞与单核细胞比例为8:1至10:1时，淋巴细胞使单核细胞产生该因子的基础能力增强了两倍。这表明淋巴细胞对LPS介导的单核细胞能力具有互补作用。LPS刺激的淋巴细胞的培养基上清液影响单核细胞的能力，而受刺激的淋巴细胞本身则无此作用。该结果表明，淋巴细胞的一种可溶性产物，即类淋巴因子介质，而非细胞实体，参与了LPS诱导的单核细胞组织因子产生过程。
