Elhaj Abubaker El, Elamin Abdalla Ahmed Eldaw, Kaplan Süleyman
Department of Histology and Embryology, Ondokuz Mayıs University, Samsun, Türkiye.
Department of Anatomy, RAK College of Medical Sciences, RAK Medical and Health Sciences University, Ras Al Khaimah, United Arab Emirates.
Histochem Cell Biol. 2025 Apr 30;163(1):48. doi: 10.1007/s00418-025-02380-7.
This research investigated the effects of benzimidazole (BZ) and electrical stimulation (ES) on peripheral nerve regeneration after short- and long-term injury and assessed functional recovery by means of stereological, histological, and electrophysiological analyses. Fifty-four male albino Wistar rats were divided into nine groups of six animals each. No treatment or surgery was applied to the control (CONT) group. The sciatic nerve was crushed for 5 s in the short-term injury (STI) and for 60 s in the long-term injury (LTI) groups. In the STI + BZ group and the LTI + BZ group, the rats received 25 mg/kg/day of BZ via oral gavage for 28 days. In the STI + ES and LTI + ES groups, a 3-V current was applied for 20 min daily for 28 days. In the STI + BZ + ES group and the LTI + BZ + ES groups, 3-V ES was applied for 20 min per day for 28 days following oral administration of BZ at 25 mg/kg/day for 28 days. All groups were subjected to electrophysiological, electron microscopic, stereological, and statistical analyses. The stereological analyses revealed a significant increases in the numbers of myelinated axons in the STI + ES groups compared with the STI (p < 0.01). BZ treatment yielded no significant differences in the numbers of myelinated axons in the groups (p > 0.05). Histological evaluation of the STI and LTI groups showed that ES and BZ treatment positively affect the histological structure of the nerve.
本研究调查了苯并咪唑（BZ）和电刺激（ES）对短期和长期损伤后周围神经再生的影响，并通过体视学、组织学和电生理分析评估功能恢复情况。54只雄性白化Wistar大鼠被分为9组，每组6只动物。对照组（CONT）不进行任何治疗或手术。在短期损伤（STI）组中，坐骨神经被挤压5秒，在长期损伤（LTI）组中被挤压60秒。在STI + BZ组和LTI + BZ组中，大鼠通过口服灌胃给予25 mg/kg/天的BZ，持续28天。在STI + ES组和LTI + ES组中，每天施加3 V电流20分钟，持续28天。在STI + BZ + ES组和LTI + BZ + ES组中，在以25 mg/kg/天的剂量口服BZ 28天后，每天施加3 V的电刺激20分钟，持续28天。所有组均接受电生理、电子显微镜、体视学和统计分析。体视学分析显示，与STI组相比，STI + ES组中有髓轴突数量显著增加（p < 0.01）。BZ治疗在各实验组的有髓轴突数量上无显著差异（p > 0.05）。STI组和LTI组的组织学评估表明，电刺激和BZ治疗对神经的组织结构有积极影响。
