Spheroid Formation and Easy and Stable Control of Stromal Cells Using Multi-inlet Spheroid Generator.

Tumor spheroids have been used as a powerful tool for testing anti-cancer drugs or understanding tumors in vivo due to their structural and physiological recapitulation of human solid tumors. Furthermore, when co-cultured with stromal cells, it is much more relevant to tumor in vivo than cancer cell alone, because stromal cells in tumor microenvironment play major roles in cancer progression and drug resistance. This protocol describes how to generate a spheroid with a controlled stromal cell ratio using a multi-inlet spheroid generator.