赞比亚小反刍兽疫病毒（PPRV）监测期间小反刍动物中犬瘟热病毒（CDV）中和抗体的检测
Detection of canine distemper virus (CDV) neutralising antibodies in small ruminants during peste-des-petits-ruminants virus (PPRV) surveillance in Zambia.
作者信息
Lysholm Sara, Logan Nicola, Lindahl Johanna F, Berg Mikael, Johansson Elin, Bergkvist Pernilla Karlsson, Dautu George, Chazya Ricky, Willett Brian J, Munyeme Musso, Wensman Jonas Johansson
机构信息
Department of Clinical Sciences, Swedish University of Agricultural Sciences, 75007, Uppsala, Sweden.
Animal and Human Health Program, Department of Biosciences, International Livestock Research Institute, Nairobi, 00100, Kenya.
出版信息
BMC Vet Res. 2025 Apr 30;21(1):303. doi: 10.1186/s12917-025-04732-w.
Canine distemper virus (CDV) is endemic in Zambia, while peste-des-petits-ruminants virus (PPRV) has not been detected. This study screened 962 farmed goats and 237 small ruminants at informal markets for PPRV antibodies using c-ELISA. A subset (n = 29) was re-analysed with virus neutralisation assays (VNA) for antibodies to PPRV and CDV due to cross-reactivity concerns. c-ELISA indicated 6.44% PPRV seroprevalence on farms and 3.80% at markets. While VNA detected no PPRV antibodies, 45% (13/29) tested positive for CDV, possibly suggesting cross-reactivity. Given the study's limitations, further research is needed to determine whether PPRV is present in Zambia and assess potential cross-reactivity.
犬瘟热病毒（CDV）在赞比亚呈地方流行性，而小反刍兽疫病毒（PPRV）尚未被检测到。本研究使用竞争酶联免疫吸附测定（c-ELISA）对962只养殖山羊和237只在非正式市场的小反刍兽进行PPRV抗体筛查。由于存在交叉反应的担忧，对一个子集（n = 29）用病毒中和试验（VNA）重新分析PPRV和CDV抗体。c-ELISA表明农场中PPRV血清阳性率为6.44%，市场中为3.80%。虽然VNA未检测到PPRV抗体，但45%（13/29）的样本CDV检测呈阳性，这可能表明存在交叉反应。鉴于该研究的局限性，需要进一步研究以确定赞比亚是否存在PPRV并评估潜在的交叉反应。
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