使用超高效液相色谱-质谱联用技术对致死性低温进行代谢组学分析。
Metabolomic analysis of fatal hypothermia using ultra-high-performance liquid chromatography‒mass spectrometry.
作者信息
Li Jia-Hao, Liu Jia-Li, Song Jian-Wen, Deng Wei-Liang, Cao Xin-Zhi, Wu Zhong-Wen, Chen Ding-Hao, Wang Hui, Yu Song, Wang Qi
机构信息
Guangzhou Key Laboratory of Forensic Multi-Omics for Precision Identification, School of Forensic Medicine, Southern Medical University, Guangzhou, Guangdong, China.
Forensic Appraisal Department, Guangdong Provincial Forensic Science of Evidence Materials (Nantian) Engineering Technology Research Center, Shenzhen, Guangdong, China.
出版信息
Front Mol Biosci. 2025 Apr 16;12:1563642. doi: 10.3389/fmolb.2025.1563642. eCollection 2025.
INTRODUCTION
The identification of fatal hypothermia remains a significant challenge in forensic medicine. Metabolomics, which reflects the overall changes in endogenous metabolites within an organism, holds substantial value in the exploration of disease mechanisms and the screening of molecular markers.
METHODS
Using ultra-high-performance liquid chromatography‒mass spectrometry (UHPLC‒MS), we conducted a metabolomic analysis of serum, heart, lung, and kidney tissues from mice with fatal hypothermia.
RESULTS
A total of 67 metabolites significantly differed across all the tissues, involving pathways such as the TCA cycle, fatty acid oxidation, arginine metabolism, histamine metabolism, and antioxidant-related pathways. Each tissue also displayed unique metabolic alterations. Additionally, we observed significant differences in the metabolomic profiles of kidney tissues from mice with different survival times.
CONCLUSION
Our findings contribute to elucidate the underlying mechanisms involved and provide a foundation for the forensic identification of markers of fatal hypothermia.
引言
在法医学中，识别致死性低温仍是一项重大挑战。代谢组学反映了生物体内内源性代谢物的整体变化，在疾病机制探索和分子标志物筛选方面具有重要价值。
方法
我们使用超高效液相色谱-质谱联用仪（UHPLC-MS），对致死性低温小鼠的血清、心脏、肺和肾脏组织进行了代谢组学分析。
结果
所有组织中共有67种代谢物存在显著差异，涉及三羧酸循环、脂肪酸氧化、精氨酸代谢、组胺代谢和抗氧化相关途径等代谢通路。每个组织也呈现出独特的代谢变化。此外，我们观察到不同存活时间的小鼠肾脏组织代谢组学图谱存在显著差异。
结论
我们的研究结果有助于阐明其中的潜在机制，并为法医学鉴定致死性低温标志物提供基础。
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