el Rassi Z, Horváth C
J Chromatogr. 1985 Jun 19;326:79-90. doi: 10.1016/s0021-9673(01)87433-1.
Rapid separation of a group of tRNAs was carried out on novel siliceous bonded stationary phases with aqueous eluents by using gradient elution with increasing or decreasing salt gradient, as usual in electrostatic interaction chromatography or hydrophobic interaction chromatography, respectively. The stationary phases consist of microparticulate macroporous silica with surface-bound polar moieties, containing weak cationic and/or hydrophobic binding sites. Depending on the nature of the binding sites, the stationary phases exhibit different retention behavior and selectivity for tRNAs. Aqueous phosphate solutions were used as the eluent, and in many cases isocratic elution was sufficient to separate seven tRNAs. Addition of magnesium ions or n-decylbetaine to the eluent resulted in lower retention, the latter causing a greater increase in the eluent strength. The optimum pH range of the eluent was 5.5-6.5.
通过使用盐梯度递增或递减的梯度洗脱，在新型硅质键合固定相上用水性洗脱剂对一组tRNA进行了快速分离，这分别是静电相互作用色谱法或疏水相互作用色谱法中常用的方法。固定相由具有表面结合极性部分的微粒大孔二氧化硅组成，含有弱阳离子和/或疏水结合位点。根据结合位点的性质，固定相对tRNA表现出不同的保留行为和选择性。磷酸盐水溶液用作洗脱剂，在许多情况下，等度洗脱足以分离七种tRNA。向洗脱剂中添加镁离子或正癸基甜菜碱会导致保留率降低，后者会使洗脱剂强度有更大的增加。洗脱剂的最佳pH范围是5.5 - 6.5。
