Bischoff R, McLaughlin L W
J Chromatogr. 1984 Dec 28;317:251-61. doi: 10.1016/s0021-9673(01)91664-4.
Modification of approximately 65% of the amine groups of an aminopropylsilyl bonded-phase silica high-performance liquid chromatographic anion exchanger (APS-Hypersil) with organic acids containing n-alkyl moieties of different chain lengths, results in mixed mode chromatographic matrices of varying hydrophobic character. These stationary phases result in high resolution of transfer ribonucleic acids (tRNAs) when used with a decreasing gradient of ammonium sulfate. The observed resolution appears to be primarily a function of interfacial precipitation effected by the high initial salt concentration of the mobile phase and the hydrophobic character of the stationary phase, followed by selective resolubilization during the decreasing salt gradient. After resolubilization, adsorptive processes may additionally contribute to resolution. The residual amine groups decrease the hydrophobic character of the stationary phase compared to a pure reversed-phase support. The mixed-mode matrices are useful for the resolution of aminoacylated tRNAs from non-aminoacylated tRNAs.
用含有不同链长正烷基部分的有机酸对氨丙基甲硅烷基键合相硅胶高效液相色谱阴离子交换剂（APS-高效硅胶）约65%的胺基进行改性，会产生具有不同疏水特性的混合模式色谱基质。当与硫酸铵梯度递减的流动相一起使用时，这些固定相会使转移核糖核酸（tRNA）实现高分辨率分离。观察到的分辨率似乎主要是由流动相的高初始盐浓度和固定相的疏水特性所导致的界面沉淀作用决定的，随后在盐梯度递减过程中进行选择性再溶解。再溶解后，吸附过程可能会对分辨率有额外贡献。与纯反相载体相比，残留的胺基降低了固定相的疏水特性。这种混合模式基质可用于从非氨酰化tRNA中分离氨酰化tRNA。
