采用高效液相色谱法分析重组人白细胞干扰素的不同形式及合成片段。

Analysis of different forms of recombinant human leukocyte interferons and synthetic fragments by high-performance liquid chromatography.

作者信息

Felix A M, Heimer E P, Lambros T J, Swistok J, Tarnowski S J, Wang C T

出版信息

J Chromatogr. 1985 Jun 26;327:359-68. doi: 10.1016/s0021-9673(01)81665-4.

Abstract

Analytical high-performance liquid chromatography (HPLC) conditions are reported for the evaluation of recombinant human interferon monomers: recombinant human leukocyte interferon A (rIFN-alpha A), rIFN-alpha D, and the hybrid rIFN-alpha A1-62/D64-166. The two monomeric forms of rIFN-alpha A (slow-migrating monomer and fast-migrating monomer) were also resolved by HPLC. Conditions are reported for the HPLC separation of oligomers (dimers and trimers) of rIFN-alpha A. The synthesis and analytical HPLC of the carboxy-terminus fragment, corresponding to IFN-alpha D (140-166), and a series of analogues comprising the IFN-alpha A (105-125) region is reported. The syntheses were accomplished by the solid-phase peptide synthesis procedure and the products were purified by preparative HPLC.

摘要

报告了用于评估重组人干扰素单体的分析型高效液相色谱（HPLC）条件：重组人白细胞干扰素A（rIFN-αA）、rIFN-αD以及杂交体rIFN-αA1-62/D64-166。rIFN-αA的两种单体形式（慢迁移单体和快迁移单体）也通过HPLC得以分离。报告了rIFN-αA低聚物（二聚体和三聚体）的HPLC分离条件。报告了对应于IFN-αD（140-166）的羧基末端片段以及一系列包含IFN-αA（105-125）区域的类似物的合成及分析型HPLC情况。合成通过固相肽合成程序完成，产物通过制备型HPLC进行纯化。