Kostrov S V, Izotova L S, Borukhov S I, Ryzhavskaia A S, Musatova A A
Antibiot Med Biotekhnol. 1986 Jan;31(1):19-23.
Highly sensitive, effective and simple methods for determination of leukocytic interferons were developed. The methods are based on formation of immune complexes of interferon with anti-interferon monoclonal and polyclonal antibodies immobilized on an insoluble basis. As a result of interferon alpha 2 purification from the biomass of the interferon-producing bacteria the preparative amounts of the protein were obtained. These amounts were sufficient for the interferon structural and physico-chemical analysis. The isolated interferons are characterized by high stability on storage and may serve a basis for preparation of pharmaceutical agents.
开发了用于测定白细胞干扰素的高灵敏度、有效且简单的方法。这些方法基于干扰素与固定在不溶性基质上的抗干扰素单克隆和多克隆抗体形成免疫复合物。通过从产生干扰素的细菌生物质中纯化α2干扰素，获得了制备量的该蛋白质。这些量足以进行干扰素的结构和物理化学分析。分离出的干扰素在储存时具有高稳定性，可作为制备药物制剂的基础。
