Gunasingham H, Tay B T, Ang K P
J Chromatogr. 1985 Jun 14;341(2):271-8. doi: 10.1016/s0378-4347(00)84040-2.
A method for the determination of oestriol in pregnancy urine by normal-phase high-performance liquid chromatography with electrochemical detection is described. A large-volume wall-jet cell with an Ag-Ag+ reference electrode was used as the detector system. The limit of detection obtained is comparable to that of electrochemical detection following reversed-phase liquid chromatography. One of the advantages of electrochemical detection with normal-phase systems is that adsorption problems are minimized.
描述了一种采用正相高效液相色谱法结合电化学检测测定孕妇尿液中雌三醇的方法。使用带有银 - 氯化银参比电极的大容量壁流池作为检测系统。所获得的检测限与反相液相色谱后的电化学检测相当。正相系统电化学检测的优点之一是吸附问题最小化。
