Shimada K, Xie F, Nambara T
J Chromatogr. 1982 Oct 8;232(1):13-8.
A high-performance liquid chromatographic method for the determination of estriol 16-glucuronide and 17-glucuronide in bile and urine has been developed. The electrochemical detector was found to be more superior than the UV detector with respect to selectivity and sensitivity and therefore more suitable for the determination of estrogen conjugates in biological fluids. The use of the present method revealed that both estriol 16-glucuronide and 17-glucuronide were excreted in rat bile, while only the former was present in human pregnancy urine.
已开发出一种用于测定胆汁和尿液中雌三醇16 - 葡萄糖醛酸苷和17 - 葡萄糖醛酸苷的高效液相色谱法。结果发现，电化学检测器在选择性和灵敏度方面优于紫外检测器，因此更适合用于测定生物体液中的雌激素结合物。使用本方法显示，雌三醇16 - 葡萄糖醛酸苷和17 - 葡萄糖醛酸苷均在大鼠胆汁中排泄，而人妊娠尿液中仅存在前者。
