Govender M, Bubb M O, Conradie J D
J Virol Methods. 1985 Jul;11(3):225-30. doi: 10.1016/0166-0934(85)90111-9.
A method is described for the rapid isolation of purified HBsAg from infected donor plasma. This method uses polyethylene glycol precipitation and a single isopycnic ultracentrifugation step in which the gradient is formed in situ. It works equally well using a small swingout rotor or a zonal rotor.