使用亲和色谱法大规模纯化乙型肝炎表面抗原。
Large-scale purification of hepatitis B surface antigen using affinity chromatography.
作者信息
Neurath A R, Prince A M, Giacalone J
出版信息
Experientia. 1978 Mar 15;34(3):414-5. doi: 10.1007/BF01923068.
PMID:631288
Abstract
Large-scale purification of hepatitis B surface antigen, applicable to the preparation of potential vaccines for prevention of hepatitis B, is described. The method involves the following steps: precipitation of serum with polyethylene glycol 6000, affinity chromatography on concanavalin A-Sepharose and on omega-aminononyl-Sepharose, and isopycnic centrifugation.
摘要
本文描述了大规模纯化乙型肝炎表面抗原的方法，该方法适用于制备预防乙型肝炎的潜在疫苗。该方法包括以下步骤：用聚乙二醇6000沉淀血清，在伴刀豆球蛋白A-琼脂糖和ω-氨壬基-琼脂糖上进行亲和层析，以及等密度离心。
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