Indrati Agnes Rengga, Kosasih Felicia Nathania, Fadhilah Fitri, Pratiwi Amelia, Muthiah Ummi, Logito Verina, Sumarpo Anton, Haryanto Jane, Munaya Shofa, Rosmiati Ni Made Dwi, Turbawaty Dewi Kartika, Wisaksana Rudi
Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran-Hasan Sadikin General Hospital, Bandung 40161, Indonesia.
Doctoral Program, Faculty of Medicine, Universitas Padjadjaran, Bandung 40135, Indonesia.
Diagnostics (Basel). 2025 Apr 10;15(8):959. doi: 10.3390/diagnostics15080959.
Approximately 10-35% of people living with HIV (PLHIV) on antiretroviral therapy (ART) fail to restore CD4 T cell counts, a state known as immunological non-responder (INR) characterized by persistent immune activation and elevated cytokine levels. : This study aimed to identify cytokines that can serve as biomarkers for immune activation and inflammation in INR patients. : We conducted a cross-sectional study comparing two groups: INRs (PLHIV on ART with viral suppression) and immunological responders (IRs). We analyzed 40 samples of virologically suppressed PLHIV, measuring CD4 T cell counts, viral load via RT-PCR, and cytokine levels through cytometric bead array (CBA). : The INR group exhibited significantly higher median serum levels of IL-6 (1.74 pg/mL vs. 0.94 pg/mL, = 0.016) and IL-10 (1.65 pg/mL vs. 0.92 pg/mL, = 0.03) compared to the IR group. : Elevated IL-6 and IL-10 levels may serve as potential markers to distinguish INR from IR patients with areas under the curve (AUC) of 0.731 and 0.707, respectively.
接受抗逆转录病毒疗法（ART）的艾滋病病毒感染者（PLHIV）中，约10%-35%的人未能恢复CD4 T细胞计数，这种状态被称为免疫无反应者（INR），其特征是持续的免疫激活和细胞因子水平升高。本研究旨在确定可作为INR患者免疫激活和炎症生物标志物的细胞因子。我们进行了一项横断面研究，比较了两组：免疫无反应者（接受ART且病毒得到抑制的PLHIV）和免疫反应者（IR）。我们分析了40份病毒得到抑制的PLHIV样本，通过逆转录聚合酶链反应（RT-PCR）测量CD4 T细胞计数和病毒载量，并通过细胞计数微球阵列（CBA）检测细胞因子水平。与IR组相比，INR组的血清白细胞介素-6（IL-6）中位数水平显著更高（1.74 pg/mL对0.94 pg/mL，P = 0.016），白细胞介素-10（IL-10）水平也显著更高（1.65 pg/mL对0.92 pg/mL，P = 0.03）。IL-6和IL-10水平升高可能作为区分INR和IR患者的潜在标志物，曲线下面积（AUC）分别为0.731和0.707。
