Indrati Agnes Rengga, Sumarpo Anton, Haryanto Jane, Rosmiati Ni Made Dwi, Munaya Shofa, Turbawaty Dewi Kartika, Wisaksana Rudi
Department of Clinical Pathology, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin Central Hospital, Bandung, West Java 40161, Indonesia.
Immunology Study Centre, Faculty of Medicine, Universitas Padjadjaran/Hasan Sadikin Central Hospital, Bandung, West Java 40161, Indonesia.
Biomed Rep. 2024 Jul 10;21(3):131. doi: 10.3892/br.2024.1819. eCollection 2024 Sep.
Individuals with human immunodeficiency virus (HIV) infection are susceptible to immune system dysregulation, particularly during co-infection with (MTB). Although there is an association between cytokine profiles and HIV-MTB co-infection, little is known about the cytokine-related host immune response mechanism to HIV-MTB co-infection. Therefore, the present study aimed to analyze expression of cytokines IL-17A, IFN-γ, TNF, IL-2, IL-10, IL-6 and IL-4 in individuals with HIV-MTB co-infection. A total of 30 patients with HIV and 40 with HIV-MTB co-infection were recruited into the present study, including those with active (A) (n=19) and latent (L)TB (n=21). HIV infection status was established based on national HIV guideline (Pedoman Nasional Pelayanan Kedokteran Tatalaksana HIV). ATB was confirmed using a positive acid-fast bacillus staining and culture of sputum; LTB status was established using IFN-γ release assay. Furthermore, the levels of cytokines IL-17A, IFN-γ, TNF, IL-10, IL-6, IL-4 and IL-2 were measured using flow cytometric bead array and CD4 cell count was performed by PIMA™ CD4 assay. IFN-γ, TNF, IL-10, IL-6 and IL-2 were able to significantly differentiate patients with HIV-ATB from those with HIV-LTB. Furthermore, in the patient subgroup with CD4 count <350 cells/µl, IFN-γ, IL-10 and IL-6 were able to differentiate between patients with HIV-ATB and HIV alone, as well as between patients with HIV-ATB and HIV-LTB. Based on these findings, the cytokine profiles are likely to be distinct between individuals with HIV infection with A- and LTB. Furthermore, the expression of CD4-positive T cells may influence the immune response in the body under HIV-MTB co-infection.
感染人类免疫缺陷病毒（HIV）的个体易发生免疫系统失调，尤其是在合并感染结核分枝杆菌（MTB）期间。尽管细胞因子谱与HIV-MTB合并感染之间存在关联，但关于细胞因子相关的宿主免疫反应机制对HIV-MTB合并感染的了解甚少。因此，本研究旨在分析HIV-MTB合并感染个体中细胞因子白细胞介素-17A（IL-17A）、干扰素-γ（IFN-γ）、肿瘤坏死因子（TNF）、白细胞介素-2（IL-2）、白细胞介素-10（IL-10）、白细胞介素-6（IL-6）和白细胞介素-4（IL-4）的表达。本研究共纳入30例HIV患者和40例HIV-MTB合并感染患者，包括活动性（A）结核患者（n=19）和潜伏性（L）结核患者（n=21）。HIV感染状态根据国家HIV指南（《印度尼西亚全国HIV治疗指南》）确定。活动性结核通过痰涂片抗酸杆菌染色阳性和培养确诊；潜伏性结核状态通过干扰素-γ释放试验确定。此外，使用流式细胞术微球阵列检测细胞因子IL-17A、IFN-γ、TNF、IL-10、IL-6、IL-4和IL-2的水平，并通过PIMA™ CD4检测法进行CD4细胞计数。IFN-γ、TNF、IL-10、IL-6和IL-2能够显著区分HIV-活动性结核患者和HIV-潜伏性结核患者。此外，在CD4计数<350个细胞/微升的患者亚组中，IFN-γ、IL-10和IL-6能够区分HIV-活动性结核患者与单纯HIV患者，以及HIV-活动性结核患者与HIV-潜伏性结核患者。基于这些发现，HIV感染合并活动性和潜伏性结核个体的细胞因子谱可能不同。此外，CD4阳性T细胞的表达可能会影响HIV-MTB合并感染时体内的免疫反应。
