Xu Chuanyun, Sun Yisha, Zhang Yang, Liu Tianqi, Wang Xiao, Hu Die, Huang Shuaiye, Li Junjie, Zhang Fanghong, Li Gang
School of Computer & Information Science, Chongqing Normal University, Chongqing 401331, China.
School of Artificial Intelligence, Chongqing University of Technology, Chongqing 401135, China.
Diagnostics (Basel). 2025 Apr 18;15(8):1032. doi: 10.3390/diagnostics15081032.
Histopathological images stained with hematoxylin and eosin (H&E) are crucial for cancer diagnosis and prognosis. However, color variations caused by differences in tissue preparation and scanning devices can lead to data distribution discrepancies, adversely affecting the performance of downstream algorithms in tasks like classification, segmentation, and detection. To address these issues, stain normalization methods have been developed to standardize color distributions across images from various sources. Recent advancements in deep learning-based stain normalization methods have shown significant promise due to their minimal preprocessing requirements, independence from reference templates, and robustness. This review examines 115 publications to explore the latest developments in this field. We first outline the evaluation metrics and publicly available datasets used for assessing stain normalization methods. Next, we systematically review deep learning-based approaches, including supervised, unsupervised, and self-supervised methods, categorizing them by core technologies and analyzing their contributions and limitations. Finally, we discuss current challenges and future directions, aiming to provide researchers with a comprehensive understanding of the field, promote further development, and accelerate the progress of intelligent cancer diagnosis.
苏木精和伊红(H&E)染色的组织病理学图像对于癌症诊断和预后至关重要。然而,由于组织制备和扫描设备的差异导致的颜色变化会导致数据分布差异,对分类、分割和检测等下游算法的性能产生不利影响。为了解决这些问题,已经开发了染色归一化方法来标准化来自各种来源图像的颜色分布。基于深度学习的染色归一化方法的最新进展因其预处理要求极低、独立于参考模板以及鲁棒性而显示出巨大的前景。本综述研究了115篇出版物,以探索该领域的最新发展。我们首先概述用于评估染色归一化方法的评估指标和公开可用的数据集。接下来,我们系统地回顾基于深度学习的方法,包括监督、无监督和自监督方法,按核心技术对它们进行分类,并分析它们的贡献和局限性。最后,我们讨论当前的挑战和未来的方向,旨在为研究人员提供对该领域的全面理解,促进进一步发展,并加速智能癌症诊断的进展。