Shen Siyi, Yuan Yuehua, Song Jinping, Zhu Yongjun, Wang Yuzhen, Yue Chaoyi, Du Mengqing, Wei Jiyuan, Feng Feng, Tian Maozhong
College of Chemistry and Chemical Engineering, Shanxi Provincial Key Laboratory of Chemical Biosensing, Shanxi Datong University, Datong 037009, PR China.
College of Chemistry and Chemical Engineering, Shanxi Provincial Key Laboratory of Chemical Biosensing, Shanxi Datong University, Datong 037009, PR China.
Food Chem. 2025 Sep 1;485:144547. doi: 10.1016/j.foodchem.2025.144547. Epub 2025 Apr 26.
As a significant reactive oxygen species (ROS), ClO plays versatile roles in daily life and many biological events. However, its abnormal levels are responsible for serious harm to human health. Therefore, it is of crucial interest to develop effective methods for detecting ClO in foods and living organisms. In this work, a novel fluorescent probe ethyl 2-(3-formyl-2-methoxy-10H-phenothiazin-10-yl)acetate (PEA) for detecting ClO was presented. It shows the merits of rapid ratiometric response (within 10 s), high selectivity and very large Stokes shift (190 nm). The detection limit of PEA for ClO was determined to be 0.47 μM. We not only successfully prepared paper test strips for efficient qualitative naked eye ClO detection, but also demonstrated its potential for the valid detection of ClO in natural water samples, beverages and foods. Furthermore, the probe PEA was also applied to the fluorescence imaging of ClO in onion epidermal cells.
作为一种重要的活性氧（ROS），次氯酸（ClO）在日常生活和许多生物过程中发挥着多种作用。然而，其异常水平会对人体健康造成严重危害。因此，开发检测食品和生物体内ClO的有效方法至关重要。在这项工作中，提出了一种用于检测ClO的新型荧光探针2-(3-甲酰基-2-甲氧基-10H-吩噻嗪-10-基)乙酸乙酯（PEA）。它具有快速比率响应（10秒内）、高选择性和非常大的斯托克斯位移（190nm）的优点。PEA对ClO的检测限确定为0.47μM。我们不仅成功制备了用于高效定性肉眼检测ClO的试纸条，还证明了其在天然水样、饮料和食品中有效检测ClO的潜力。此外，探针PEA还应用于洋葱表皮细胞中ClO的荧光成像。
