Bin Yidong, Huang Lixian, Qin Jiangke, He Min, Zhao Shulin, Huang Yong, Zhao Jingjin
State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education of China), Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin 541004, China.
Anal Chem. 2025 May 13;97(18):9798-9808. doi: 10.1021/acs.analchem.4c07025. Epub 2025 May 2.
Sulfur dioxide (SO) dysmetabolism is closely associated with various diseases such as acute kidney injury (AKI). Nevertheless, the relationship between SO levels and iodinated contrast-induced AKI remains largely unclear. Therefore, accurate imaging of SO level fluctuations is therefore critically important. However, no photoacoustic (PA) imaging method is currently available for the detection of SO. To address this gap, we designed and synthesized a PA probe toward SO, namely, Rho-QL, for the first time and performed in situ PA imaging of SO in deep tissues . A novel method was accordingly developed for the 3D visual diagnosis of AKI based on PA imaging of SO in kidney tissues with high spatial resolution. Rho-QL exhibited a PA response time of 5 s for SO and displayed remarkable turn-on absorption changes at 700 nm, making it a suitable probe for detecting SO levels via PA imaging. Moreover, Rho-QL exhibited an excellent targeting ability to the kidney, thereby facilitating imaging of SO in the kidney. Notably, through real-time PA imaging, Rho-QL was successfully applied for 3D visualization of the detailed SO distribution with high spatial resolution and revealed a remarkable increase in the SO levels in the kidney during a contrast-induced AKI process. Based on the current findings, Rho-QL is expected to become a powerful tool for the study and diagnosis of AKI-related diseases.
二氧化硫（SO）代谢紊乱与急性肾损伤（AKI）等多种疾病密切相关。然而，SO水平与碘化造影剂诱导的AKI之间的关系仍不清楚。因此，准确成像SO水平波动至关重要。然而，目前尚无用于检测SO的光声（PA）成像方法。为填补这一空白，我们首次设计并合成了一种针对SO的PA探针，即Rho-QL，并对深部组织中的SO进行了原位PA成像。据此，基于对肾组织中SO的高空间分辨率PA成像，开发了一种用于AKI三维可视化诊断的新方法。Rho-QL对SO的PA响应时间为5秒，在700 nm处显示出显著的开启吸收变化，使其成为通过PA成像检测SO水平的合适探针。此外，Rho-QL对肾脏表现出优异的靶向能力，从而便于对肾脏中的SO进行成像。值得注意的是，通过实时PA成像，Rho-QL成功应用于高空间分辨率的SO详细分布的三维可视化，并揭示了在造影剂诱导的AKI过程中肾脏中SO水平的显著增加。基于目前的研究结果，Rho-QL有望成为研究和诊断AKI相关疾病的有力工具。
