Guoqing Chen, Chunxiang Li, Qian Cui, Changcheng Li, Pengfei Yang, Qingli Yan, Ran An, Wei Liu, Feng Li, Kuikui Lu, Hongjun Zhang, Haiyan Peng
Department of Pathogenic Microbiology and Biological Laboratory, Yancheng Municipal Center for Disease Control and Prevention, Yancheng, Jiangsu, China.
Laboratory Department, Yancheng Maternal and Child Health Hospital, Yancheng, Jiangsu, China.
J Trop Med. 2025 Apr 23;2025:9926259. doi: 10.1155/jotm/9926259. eCollection 2025.
To investigate the infection status, species composition, and genetic diversity of in local rodent populations in coastal mudflat wetland habitats in eastern Jiangsu Province of China. From March to June 2023, rodents were captured in mudflat wetlands of Dongtai and Tinghu Counties, Eastern China. Rodent species were identified, and nucleic acids were extracted from liver and spleen tissues. The mitochondrial cytochrome b (mt-) gene was amplified by PCR, while -specific citrate synthase () and 16S rRNA genes were amplified by semi-nested PCR. Phylogenetic and homology analyses were conducted to identify rodent and species. Among 29 captured rodents, 26 were and 3 were . Phylogenetic analysis of the mt- gene divided into 7 lineages, each linked to geographically diverse populations. Six rodents tested positive for , with a positivity rate of 20.69%. Phylogenetic analyses revealed three species: , , and one undetermined species. The infected strains clustered into three evolutionary branches based on and 16S rRNA genes. This study provides the first evidence of infection among rodent populations in wetland habitats along China's eastern coast. The region harbors diverse rodent species, with a high infection rate, and at least three species were identified, including a potential novel species.
为调查中国江苏省东部沿海泥滩湿地生境中当地啮齿动物种群的感染状况、种类组成和遗传多样性。2023年3月至6月，在中国东部东台和亭湖两县的泥滩湿地捕获啮齿动物。鉴定啮齿动物种类，并从肝脏和脾脏组织中提取核酸。通过聚合酶链反应（PCR）扩增线粒体细胞色素b（mt-）基因，同时通过半巢式PCR扩增特定柠檬酸合酶（）和16S核糖体RNA基因。进行系统发育和同源性分析以鉴定啮齿动物和种类。在捕获的29只啮齿动物中，26只为，3只为。对mt-基因的系统发育分析将分为7个谱系，每个谱系与地理上不同的种群相关。6只啮齿动物检测呈阳性，阳性率为20.69%。系统发育分析揭示了三种种类：、和一种未确定的种类。基于和16S rRNA基因，感染的菌株聚为三个进化分支。本研究首次提供了中国东部沿海湿地生境中啮齿动物种群感染的证据。该地区有多种啮齿动物，感染率高，且鉴定出至少三种种类，包括一种潜在的新种类。
