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在中国东部的啮齿动物中检测到的物种。

Species Detected in Rodents from Eastern China.

机构信息

State Key Laboratory of Virology, School of Health Sciences, Wuhan University, Wuhan, Hubei Province, China.

School of Medicine, Fudan University, Shanghai, China.

出版信息

Vector Borne Zoonotic Dis. 2019 Nov;19(11):810-814. doi: 10.1089/vbz.2018.2410. Epub 2019 Jul 29.

Abstract

are vector borne gram-negative facultative intracellular bacteria. species are associated with rodents and their flea parasites worldwide. The genetic variation and distribution of species in rodents are not clear in China. We investigated the presence and genetic diversity of species in rodents from eastern China. We captured rodents from 2015 to 2016 in Jiaonan County, Shandong Province, and detected species in the spleen of rodents by PCR amplification of the citrate synthase () gene and RNA polymerase beta subunit () gene. We found that 8.38% (16/191) of the rodents were positive by PCR for both and genes; that sequences from the rodents were phylogenetically divided into five clades, which were closely related to , , , , and , respectively; and that each species is rodent species-specific with and for , for , for , and for . This study indicated that organisms have a broad distribution and a variety of genotypes in rodents in eastern China and the threats to public health by these species should be monitored in China.

摘要

是一种经媒介传播的革兰氏阴性兼性细胞内细菌。该属的物种与世界各地的啮齿动物及其跳蚤寄生虫有关。在中国,关于啮齿动物中 物种的遗传变异和分布尚不清楚。我们调查了来自中国东部的啮齿动物中 物种的存在和遗传多样性。我们于 2015 年至 2016 年在山东省胶南市捕获啮齿动物,并通过柠檬酸合酶()基因和 RNA 聚合酶β亚基()基因的 PCR 扩增检测啮齿动物脾脏中的 物种。我们发现,8.38%(16/191)的啮齿动物的 和 基因 PCR 均为阳性;来自啮齿动物的 序列在系统发育上分为五个分支,分别与 、 、 、 和 密切相关;每个 物种都是啮齿动物种特异性的, 用于 , 用于 , 用于 , 用于 。本研究表明,在中国东部的啮齿动物中, 生物体具有广泛的分布和多种基因型,应监测这些 物种对公众健康的威胁。

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