Conlon P J, Hepper K P, Teresa G W
Infect Immun. 1977 Feb;15(2):510-7. doi: 10.1128/iai.15.2.510-517.1977.
Two strains of mice, Swiss Webster and DBA/2Cr, were injected intraperitoneally or intravenously with varying dosages of Fusobacterium necrophorum. The ability to eliminate the infection was assessed by quantitative enumeration of the organisms present in the blood, liver, and spleen, Three- to 4-week-old DBA/2Cr mice were highly resistant to both routes of injection. The intraperitoneal injection of older mice failed to demonstrate a dose-effect relationship whereas an intravenous injection of as few as 10(4) cells of F. necrophorum produced progressively necrotic leg abscesses, apparently involving the lymphonodus ischiadicus which filters the site of injection. Mortality was increased with sensitization by a previous sublethal injection. Also, an ethanol-killed cell vaccine delayed the onset of lethal infection, whereas repeated sublethal live cell injections provided nonspecific protection since mice vaccinated with the growth medium were equally protected. The development of leg abscesses after intravenous injection visibly demonstrated the pathogenicity of F. necrophorum and may provide a suitable model for the evaluation of vaccines and the effectiveness of antibiotics.
将两株小鼠，即瑞士韦伯斯特小鼠和DBA/2Cr小鼠，通过腹腔内或静脉内注射不同剂量的坏死梭杆菌。通过对血液、肝脏和脾脏中存在的微生物进行定量计数来评估消除感染的能力。3至4周龄的DBA/2Cr小鼠对两种注射途径都具有高度抗性。对老龄小鼠进行腹腔内注射未能显示出剂量效应关系，而静脉内注射低至10⁴个坏死梭杆菌细胞会产生逐渐坏死的腿部脓肿，显然涉及过滤注射部位的坐骨淋巴结。通过先前的亚致死注射致敏会增加死亡率。此外，乙醇灭活细胞疫苗可延迟致死性感染的发作，而重复的亚致死活细胞注射提供非特异性保护，因为用生长培养基接种的小鼠受到同等保护。静脉注射后腿部脓肿的形成明显证明了坏死梭杆菌的致病性，并且可能为评估疫苗和抗生素的有效性提供合适的模型。
