Teke Halenur, Balci Senay, Neselioglu Salim, Teke Selçuk, Erel Ozcan, Tamer Lulufer, Toros Fevziye
Department of Child and Adolescent Psychiatry, Medical Faculty, Mersin University, Ankara, Turkey.
Department of Medical Biochemistry, Medical Faculty, Mersin University, Mersin, Turkey.
J Mol Neurosci. 2025 May 2;75(2):62. doi: 10.1007/s12031-025-02358-z.
Autism spectrum disorder (ASD) is a complex neurodevelopmental condition with multifactorial etiopathogenesis, where oxidative stress (OS) has been implicated as a key contributing factor. This study aimed to evaluate the plasma dynamic thiol/disulfide homeostasis (DTDH) parameters-a relatively novel OS biomarker-alongside classical OS biomarkers, including total oxidant status (TOS), total antioxidant status (TAS), oxidative stress index (OSI), glutathione, and glutathione peroxidase (GPx), in preschool children diagnosed with ASD. A total of 49 children with ASD and 31 age- and sex-matched typically developing children between the ages of 2 and 6 years were included. In addition to sociodemographic data collection, the Childhood Autism Rating Scale (CARS) and Clinical Global Impression-Severity Scale (CGI-S) were administered to assess autism severity. Blood samples were analyzed using automated spectrophotometric techniques to determine OS biomarkers. The results demonstrated that DTDH parameters and classical OS markers exhibited parallel changes; however, no statistically significant differences were detected between the ASD and control groups across all OS markers. Furthermore, no significant association was found between OS biomarkers and autism severity. Moreover, we intentionally restricted our sample to a younger age group to enable a focused examination of OS dynamics during early developmental stages. This study underscores the potential impact of age as a critical determinant in OS-related alterations in autism and highlights the need for further age-stratified investigations to elucidate the role of OS in ASD pathophysiology and its potential diagnostic relevance.
自闭症谱系障碍（ASD）是一种具有多因素病因发病机制的复杂神经发育疾病，其中氧化应激（OS）被认为是一个关键的促成因素。本研究旨在评估血浆动态硫醇/二硫键稳态（DTDH）参数——一种相对新颖的OS生物标志物——以及经典的OS生物标志物，包括总氧化剂状态（TOS）、总抗氧化剂状态（TAS）、氧化应激指数（OSI）、谷胱甘肽和谷胱甘肽过氧化物酶（GPx），这些指标来自被诊断为ASD的学龄前儿童。总共纳入了49名患有ASD的儿童和31名年龄和性别匹配、年龄在2至6岁之间的发育正常儿童。除了收集社会人口统计学数据外，还使用儿童自闭症评定量表（CARS）和临床总体印象严重程度量表（CGI-S）来评估自闭症的严重程度。使用自动分光光度技术分析血样以确定OS生物标志物。结果表明，DTDH参数和经典OS标志物呈现平行变化；然而，在所有OS标志物方面，ASD组和对照组之间未检测到统计学上的显著差异。此外，未发现OS生物标志物与自闭症严重程度之间存在显著关联。此外，我们有意将样本限制在较年轻的年龄组，以便能够重点检查早期发育阶段的OS动态。本研究强调了年龄作为自闭症中OS相关改变的关键决定因素的潜在影响，并强调需要进一步进行年龄分层调查，以阐明OS在ASD病理生理学中的作用及其潜在的诊断相关性。
