Uengwetwanit Tanaporn, Uawisetwathana Umaporn, Angthong Pacharaporn, Phanthura Mongkhol, Phromson Metavee, Tala Siriporn, Thepsuwan Timpika, Chaiyapechara Sage, Prathumpai Wai, Rungrassamee Wanilada
Biosensing and Bioprospecting Technology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 111 Thailand Science Park, Phahonyothin Road, Khlong Nueng, 12120, Khlong Luang, Pathum Thani, Thailand.
International Joint Research Center on Food Security (IJC-FOODSEC), 111 Thailand Science Park, Phahonyothin Road, 12120, Khlong Luang, Pathum Thani, Thailand.
Sci Rep. 2025 May 2;15(1):15377. doi: 10.1038/s41598-025-00157-5.
β-glucans supplements are known for enhancing disease resistance and performance in shrimp aquaculture, but their effectiveness depends on their source and structure. This study investigated a novel β-glucan derived from the insect fungus Ophiocordyceps dipterigena BCC 2073 as a potential feed additive for Pacific white shrimp (Penaeus vannamei). To determine its feasibility, juvenile shrimps were fed diets supplemented with 0%, 0.1%, and 0.4% β-glucan oligosaccharide for 30 days prior to their performance evaluation. The groups with β-glucan supplementation showed improved immune levels and significantly higher survival rates (p-value < 0.05) when exposed to the pathogen Vibrio harveyi. Transcriptome, microbiome, and metabolome were employed to understand mechanisms of β-glucan supplement. The feed additive altered the expression of host genes linked to immunity, inflammation, and intestinal barrier function. Moreover, Vibrio spp. and Pseudoalteromonas spp. abundances were significantly modulated (p-value < 0.05) with specific Vibrio clades responding differently depending on the β-glucan concentration. Metabolomic analysis revealed immune-supporting metabolites such as hydroquinone and nicotinic acid, potentially promoting homeostasis, consistent with the observed gene expression profiles. This study highlights the potential of O. dipterigena BCC 2073 β-glucan as a novel feed additive to improve disease resistance and shrimp health.
β-葡聚糖补充剂在对虾养殖中因能增强抗病能力和提高生长性能而闻名，但其效果取决于其来源和结构。本研究调查了一种源自昆虫真菌双翅虫草Ophiocordyceps dipterigena BCC 2073的新型β-葡聚糖，作为凡纳滨对虾（Penaeus vannamei）潜在的饲料添加剂。为确定其可行性，在对幼虾进行性能评估前，先给它们投喂添加了0%、0.1%和0.4%β-葡聚糖寡糖的饲料30天。添加β-葡聚糖的组在接触哈维氏弧菌病原体时，免疫水平提高，存活率显著更高（p值<0.05）。利用转录组学、微生物组学和代谢组学来了解β-葡聚糖补充剂的作用机制。这种饲料添加剂改变了与免疫、炎症和肠道屏障功能相关的宿主基因表达。此外，弧菌属和假交替单胞菌属的丰度受到显著调节（p值<0.05），特定弧菌分支对β-葡聚糖浓度的反应不同。代谢组学分析揭示了对免疫有支持作用的代谢物，如水杨酸和烟酸，可能促进体内平衡，这与观察到的基因表达谱一致。本研究突出了双翅虫草O. dipterigena BCC 2073β-葡聚糖作为一种新型饲料添加剂来提高抗病能力和对虾健康水平的潜力。
