Dan Liu, Kang-Zheng Lee
Bioprocessing Technology Institute BTI, Agency for Science, Technology and Research (A*STAR), 20 Biopolis Way, Singapore, 138668, Singapore.
J Transl Med. 2025 May 2;23(1):501. doi: 10.1186/s12967-025-06524-0.
Immune cell therapies have revolutionized the treatment of cancers, autoimmune disorders, and infectious diseases. A critical step in their manufacturing is viral transduction, which enables the delivery of therapeutic genes into immune cells. However, the complexity of this process presents significant challenges for optimization and scalability. This review provides a comprehensive analysis of viral transduction process in immune cell therapy manufacturing, highlighting key design considerations to support the development of safe, effective, and scalable production methods. Additionally, it examines current technological challenges in immune cell transduction and explores future innovations poised to advance the field.
免疫细胞疗法彻底改变了癌症、自身免疫性疾病和传染病的治疗方式。其生产过程中的一个关键步骤是病毒转导，它能将治疗性基因导入免疫细胞。然而，这一过程的复杂性给优化和扩大规模带来了重大挑战。本文综述对免疫细胞治疗生产中的病毒转导过程进行了全面分析，强调了关键的设计考量因素，以支持安全、有效且可扩展的生产方法的开发。此外，还研究了免疫细胞转导当前的技术挑战，并探索了有望推动该领域发展的未来创新。
