Yadav Ranu, Yadav Santosh Kumar, Mishra Disha, Khare Puja
Crop Production and Protection Division, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226015, India.
Academy of Scientific and Innovative Research AcSIR, Ghaziabad, 201002, India.
Biodegradation. 2025 May 3;36(3):37. doi: 10.1007/s10532-025-10135-3.
The eleven combinations of four isolates, S. maltophilia, P. hibiscicola, P. aeruginosa, and P. monteilii, were prepared and screened for chlorpyrifos (CP) degradation. Among these combinations, four highly CP degrading consortia were identified: D: S. maltophilia, P. hibiscicola, P. monteilii, E: P. hibiscicola, P. aeruginosa, P. monteilii, F: S. maltophilia, P. hibiscicola, and G: S. maltophilia, P. aeruginosa. These combinations were found to be mutually compatible, exhibiting no lysis or inhibition zones. Their application significantly decreased in CP content from 37.6 to 68.6% as compared to control. Consortia-treated soil also displayed reduced bio-concentration factor and translocation of CP in W. somnifera. A significant increase in biomass (40-71.2%), protein content (38-66.6%), chlorophyll (24.7-52.3%), and secondary metabolite of W. somnifera was observed after the application of consortia. The activities of soil enzymes (alkaline phosphatase, dehydrogenase, and N-acetyl glucosaminidase), availability of nutrients, and soil microbial biomass carbon were also enhanced by the inoculation of consortia in soil. Overall, the results indicated that the consortium of S. maltophilia and P. aeruginosa exhibited the highest potential for CP degradation and plant growth promotion compared to the others. This consortium could be effectively utilized for the rapid degradation of CP in agricultural soil vis-a-vis improvement in the productivity of the plants.
制备了嗜麦芽窄食单胞菌、木槿栖假单胞菌、铜绿假单胞菌和蒙特利假单胞菌这四种分离菌的11种组合，并对其进行毒死蜱（CP）降解筛选。在这些组合中，鉴定出四个高效CP降解菌群：D：嗜麦芽窄食单胞菌、木槿栖假单胞菌、蒙特利假单胞菌；E：木槿栖假单胞菌、铜绿假单胞菌、蒙特利假单胞菌；F：嗜麦芽窄食单胞菌、木槿栖假单胞菌；G：嗜麦芽窄食单胞菌、铜绿假单胞菌。发现这些组合相互兼容，未出现裂解或抑制区。与对照相比，它们的应用使CP含量显著降低了37.6%至68.6%。经菌群处理的土壤中，CP在睡茄中的生物富集系数和转运也有所降低。施用菌群后，观察到睡茄的生物量（40 - 71.2%）、蛋白质含量（38 - 66.6%）、叶绿素（24.7 - 52.3%）和次生代谢产物显著增加。在土壤中接种菌群还提高了土壤酶（碱性磷酸酶、脱氢酶和N - 乙酰葡糖胺酶）的活性、养分有效性以及土壤微生物生物量碳。总体而言，结果表明，与其他菌群相比，嗜麦芽窄食单胞菌和铜绿假单胞菌的菌群在CP降解和促进植物生长方面具有最高潜力。该菌群可有效用于农业土壤中CP的快速降解以及植物生产力的提高。
