Budowle B, Chow G H
J Forensic Sci. 1985 Jul;30(3):893-7.
A routine method is described for obtaining reproducible haptoglobin patterns from bloodstains by discontinuous polyacrylamide gel electrophoresis. By employing a stacking gel, proteins from bloodstain extracts are concentrated into narrow zones, before entering the resolving gel. This effect yields highly resolved haptoglobin patterns. Therefore, laboratories without the specialized equipment and expertise for polyacrylamide gradient gel preparation can still obtain highly resolved haptoglobin patterns from bloodstains.
本文描述了一种常规方法，通过不连续聚丙烯酰胺凝胶电泳从血迹中获得可重复的触珠蛋白图谱。通过使用堆积凝胶，血迹提取物中的蛋白质在进入分离凝胶之前被浓缩成狭窄的区域。这种效果产生了高度分辨的触珠蛋白图谱。因此，没有制备聚丙烯酰胺梯度凝胶的专业设备和专业知识的实验室仍然可以从血迹中获得高度分辨的触珠蛋白图谱。
