Budowle B, Scott E
Forensic Sci Int. 1985 Aug;28(3-4):269-75. doi: 10.1016/0379-0738(85)90136-7.
A method was described for subtyping transferrin derived from human bloodstains. Bloodstain cuttings were extracted in 0.5% ferrous ammonium sulfate. The extracts were subjected to ultrathin-layer polyacrylamide gel isoelectric focusing. After isoelectric focusing, transferrin was detected by silver staining. This method permitted the successful typing of Tf in 6-month-old blood stains maintained at -20 degrees C and room temperature and 3-month-old bloodstains maintained at 37 degrees C.
描述了一种对源自人类血迹的转铁蛋白进行亚型分型的方法。将血迹切片在0.5%硫酸亚铁铵中提取。提取物进行超薄层聚丙烯酰胺凝胶等电聚焦。等电聚焦后，通过银染检测转铁蛋白。该方法能够成功对保存在-20℃和室温下6个月的血迹以及保存在37℃下3个月的血迹中的转铁蛋白进行分型。
