Heydarzadeh Shabnam, Moshtaghie Ali Asghar, Daneshpour Maryam, Hedayati Mehdi
Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Molecular Biology, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran.
Discov Oncol. 2025 May 3;16(1):666. doi: 10.1007/s12672-025-02339-z.
Owing to impaired iodine metabolism, anaplastic thyroid carcinoma, recognized as one of the most formidable malignancies, is resistant to radioiodine therapy. This study was conducted to determine the possible role of Genistein as plant secondary metabolite in understanding the effectiveness of this nutraceutical compound in modulating Sodium iodide symporter (NIS) expression and iodide uptake in the SW1736 anaplastic thyroid cancer cell linen.
Cell viability was assessed using the MTT assay, whereas cell apoptosis was assessed using a flow cytometry assay (Annexin V-FITC Apoptosis Detection kit). To determine iodide uptake by SW1736 cells, a Sandell-Kolthoff reaction-based spectrophotometric assay was performed. QRT-PCR and western blotting were used to measure NIS mRNA and protein levels in SW1736 cells.
SW1736 cells exhibited time- and dose-dependent growth inhibition in response to Genistein treatment. QRT-PCR analysis revealed that NIS mRNA levels in the Genistein-treated group were significantly (p < 0.05) higher than those in the non-treated group. Accordingly, Western blot analysis revealed that SW1736 cells treated with Genistein expressed a high level of the NIS protein (p < 0.01). Compared with the control group, Genistein increased iodide uptake (p < 0.001) in the thyroid cancer cell line SW1736.
The expression of NIS and subsequent iodine uptake after Genistein treatment suggest that ATC cells may be becoming more like differentiated thyroid cells, which typically have higher iodine uptake capabilities. However, this redifferentiation does not directly correlate with an increase in sensitivity to radioactive iodine treatment.
由于碘代谢受损，间变性甲状腺癌被认为是最可怕的恶性肿瘤之一，对放射性碘治疗具有抗性。本研究旨在确定染料木黄酮作为植物次生代谢产物在理解这种营养化合物调节SW1736间变性甲状腺癌细胞系中碘化钠同向转运体（NIS）表达和碘摄取有效性方面的可能作用。
使用MTT法评估细胞活力，而使用流式细胞术检测（膜联蛋白V-FITC凋亡检测试剂盒）评估细胞凋亡。为了确定SW1736细胞对碘的摄取，进行了基于桑德尔-科尔托夫反应的分光光度法检测。采用实时定量聚合酶链反应（QRT-PCR）和蛋白质免疫印迹法检测SW1736细胞中NIS的mRNA和蛋白质水平。
SW1736细胞对染料木黄酮处理表现出时间和剂量依赖性的生长抑制。QRT-PCR分析显示，染料木黄酮处理组的NIS mRNA水平显著高于未处理组（p<0.05）。相应地，蛋白质免疫印迹分析显示，经染料木黄酮处理的SW1736细胞表达高水平的NIS蛋白（p<0.01）。与对照组相比，染料木黄酮增加了甲状腺癌细胞系SW1736中的碘摄取（p<0.001）。
染料木黄酮处理后NIS的表达及随后的碘摄取表明，间变性甲状腺癌细胞可能变得更像分化型甲状腺细胞，后者通常具有更高的碘摄取能力。然而，这种再分化与对放射性碘治疗敏感性的增加没有直接关联。
