Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four genes in tandem on an IncFII plasmid from . Whole-genome sequencing (WGS) of isolates L710hy identified a four-tandem repeat of the gene, with each copy's environment nearly identical (IS--IS- -------IS). The study suggests a correlation between the copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four tandem repeat structures was also investigated. This is the first documented case of tandem repeats in , and the IS and other insertion sequence may play a role in this arrangement.