首次对携带于 IncFII 质粒上的四个重复区域进行特征分析。
First characterization of four repeat regions with the carried on an IncFII plasmid in .
作者信息
Liu Yi, Liu Xiaojing, Liu Ruishan, Xu Hao, Chen Mantao, Qian Jiajie, Zheng Beiwen
机构信息
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Shandong First Medical University and Shandong Academy of Medical Sciences, Jinan, China.
出版信息
iScience. 2025 Apr 8;28(5):112369. doi: 10.1016/j.isci.2025.112369. eCollection 2025 May 16.
Carbapenem-resistant Enterobacteriaceae (CRE) have emerged as a critical threat to global health, and multicopy resistance genes are a potential contributor to increased resistance. This study investigates a novel arrangement of four genes in tandem on an IncFII plasmid from . Whole-genome sequencing (WGS) of isolates L710hy identified a four-tandem repeat of the gene, with each copy's environment nearly identical (IS--IS- -------IS). The study suggests a correlation between the copy number and minimum inhibitory concentration (MIC) values for carbapenems, though further research is needed to confirm this correlation. The stability of these four tandem repeat structures was also investigated. This is the first documented case of tandem repeats in , and the IS and other insertion sequence may play a role in this arrangement.
耐碳青霉烯类肠杆菌科细菌（CRE）已成为对全球健康的重大威胁，多拷贝耐药基因是耐药性增加的一个潜在因素。本研究调查了来自……的IncFII质粒上四个基因串联的一种新排列。分离株L710hy的全基因组测序（WGS）鉴定出该基因的四个串联重复序列，每个拷贝的环境几乎相同（IS--IS- -------IS）。该研究表明碳青霉烯类药物的拷贝数与最低抑菌浓度（MIC）值之间存在相关性，不过还需要进一步研究来证实这种相关性。还对这四个串联重复结构的稳定性进行了研究。这是……中首次记录的串联重复案例，IS和其他插入序列可能在这种排列中起作用。
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