Liu Huiqiong, Wang Dao, Tang Miaomiao, Jia Peisheng, Huo Yufeng, Wei Erhu, Xu Hao, Chi Xiaohui, Wang Huaili
Department of Pediatric Intensive Care Unit, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, People's Republic of China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, People's Republic of China.
Infect Drug Resist. 2022 Aug 31;15:5035-5042. doi: 10.2147/IDR.S367073. eCollection 2022.
With the spread of multiple drug-resistant bacteria, and have been detected in various bacteria worldwide. However, the simultaneous detection of and in has been rarely reported. This study identified an strain carrying both and . We investigated the genetic characteristics of these two resistance genes in detail, elucidating various potential mechanisms by which they may be transmitted.
Bacterial genomic features and possible origins were assessed by whole-genome sequencing (WGS) with Illumina and PacBio platforms and phylogenetic analysis. Subsequent investigations were performed, including antimicrobial susceptibility testing and multilocus sequence typing (MLST).
We isolated an strain DY1901 carrying both and from the sputum sample. Susceptibility testing showed that the isolate was multidrug-resistant. Multiple antibiotic resistance genes and virulence genes are widely distributed in DY1901. S1-PFGE, Southern blotting, and plasmid replicon typing showed that DY1901 carried four plasmids. The plasmid carrying was 259Kb in size and belonged to IncHI2, while the plasmid carrying was 45Kb in length and belonged to IncX3.
The strain isolated in this study has a broad antibiotic resistance spectrum, posing a challenge to clinical treatment. Plasmids carrying are fusion plasmids, and those taking NDM are widely disseminated in China, suggesting that we should conduct routine genomic surveillance on such plasmids to curb the spread of drug-resistant bacteria in the region.
随着多重耐药菌的传播，[相关耐药基因]已在全球各种细菌中被检测到。然而，在[特定细菌]中同时检测到[两种耐药基因]的情况鲜有报道。本研究鉴定出一株同时携带[两种耐药基因]的[细菌名称]菌株。我们详细研究了这两种耐药基因的遗传特征，阐明了它们可能传播的各种潜在机制。
通过Illumina和PacBio平台的全基因组测序（WGS）及系统发育分析评估细菌基因组特征和可能的来源。随后进行了包括抗菌药物敏感性测试和多位点序列分型（MLST）在内的调查。
我们从痰液样本中分离出一株同时携带[两种耐药基因]的[细菌名称]菌株DY1901。药敏试验表明该分离株具有多重耐药性。多种抗生素耐药基因和毒力基因在DY1901中广泛分布。S1-PFGE、Southern印迹和质粒复制子分型显示DY1901携带4种质粒。携带[一种耐药基因]的质粒大小为259Kb，属于IncHI2型，而携带[另一种耐药基因]的质粒长度为45Kb，属于IncX3型。
本研究分离出的[细菌名称]菌株具有广泛的抗生素耐药谱，给临床治疗带来挑战。携带[一种耐药基因]的质粒是融合质粒，携带NDM的质粒在中国广泛传播，这表明我们应对此类质粒进行常规基因组监测，以遏制该地区耐药菌的传播。
