Cagini C, Spinosi M, Della Lena F, Carafa M P, Marini D, Russo C, Pietrella D
Department of Medicine and Surgery, Ophthalmology Section, S. Maria della Misericordia Hospital, University of Perugia, Perugia, Italy. e-mail:
Eur Rev Med Pharmacol Sci. 2025 Apr;29(4):160-173. doi: 10.26355/eurrev_202504_37163.
OBJECTIVE: The aim of the study was to evaluate the in vitro antiseptic power of nine ophthalmic preparations containing active ingredients with known antimicrobial properties: Polyhexamethylene biguanide 0.0003%; Hexamidine diisethionate 0.1%; chlorhexidine digluconate 0.02%; Povidone-iodine 0.6%; Hexamidine diisethionate 0.05% and polyhexanide hydrochloride 0.0001%; Hypochlorous acid 0.011%; Biosecur 2 g/100 mL; Self-preserved ozonated vegetable oil; Polyquaternium-133 0.1%. MATERIALS AND METHODS: Organisms were selected based on the current review of endophthalmitis literature: Staphylococcus aureus (ATCC 29213), Staphylococcus epidermidis (ATCC 35984), Pseudomonas aeruginosa (ATCC 15692) and Candida albicans (SC5314). The effect of the eye drops on bacterial cultures and yeast cells was evaluated using the Minimum Inhibitory Concentration assay and growth kinetics analysis. RESULTS: All tested ophthalmic solutions demonstrated antiseptic activity by reducing the microbial load of the selected organisms in vitro. At higher dilutions, some formulations maintained antiseptic properties. CONCLUSIONS: The antiseptic power of ophthalmic preparations tested in vitro is effective against species of microorganisms responsible for eye infections. Polyquaternium-133 0.1% has shown the best antiseptic activity, proving to inhibit or slow down the growth of microorganisms even at low concentrations.
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目的：本研究旨在评估九种含有已知抗菌特性活性成分的眼科制剂的体外抗菌能力：0.0003%的聚六亚甲基双胍；0.1%的双碘喹啉；0.02%的葡萄糖酸氯己定；0.6%的聚维酮碘；0.05%的双碘喹啉和0.0001%的聚己内酰胺盐酸盐；0.011%的次氯酸；2 g/100 mL的Biosecur；自保存的臭氧化植物油；0.1%的聚季铵盐-133。材料与方法：根据当前眼内炎文献综述选择菌株：金黄色葡萄球菌（ATCC 29213）、表皮葡萄球菌（ATCC 35984）、铜绿假单胞菌（ATCC 15692）和白色念珠菌（SC5314）。使用最低抑菌浓度测定法和生长动力学分析评估滴眼液对细菌培养物和酵母细胞的作用。结果：所有测试的眼科溶液通过在体外降低所选菌株的微生物载量均表现出抗菌活性。在较高稀释度下，一些制剂仍保持抗菌特性。结论：体外测试的眼科制剂的抗菌能力对引起眼部感染的微生物种类有效。0.1%的聚季铵盐-133表现出最佳的抗菌活性，即使在低浓度下也能抑制或减缓微生物的生长。
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