Wang Lei, Huang Yiwen, Wu Yajuan, Huang Yuanbo, Lu Zhou, Wu Panyu, Meng Shuo, Tian Tian, Cao Lei
Department of Dermatology, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, China.
Department of Neurobiology, Nanjing Medical University, Nanjing, Jiangsu, China.
Platelets. 2025 Dec;36(1):2498353. doi: 10.1080/09537104.2025.2498353. Epub 2025 May 5.
The application of platelet concentrates such as platelet-rich plasma (PRP) for the treatment of hair loss is impeded by the absence of standardized preparation protocols and storage conditions. Providing off-the-shelf controlled product methods and inheriting regenerative function of platelets, platelet-derived extracellular vesicles (PEVs) can be a promising alternative to surpass platelet concentrates limitations. This study aimed to investigate the effects of PEVs on cultured dermal papilla cells (DPCs) and hair follicles (HFs) in animal model. PEVs were prepared from fresh apheresis platelets and characterized. Their impact on the proliferation and migration of DPCs was explored. After subcutaneous administration of PEVs in a mouse model of depilation-induced hair regeneration, we observed the accelerated transition of HFs from the telogen to the anagen phase, and found the β-catenin signaling pathway was involved. These results indicate that PEVs activate DPCs and promote HF growth effectively. The PEVs from apheresis platelets offer a robust alternative for the treatment of hair loss.
诸如富血小板血浆（PRP）等血小板浓缩物在治疗脱发方面的应用因缺乏标准化的制备方案和储存条件而受到阻碍。血小板衍生的细胞外囊泡（PEVs）能够提供现成的可控产品方法并保留血小板的再生功能，有望成为克服血小板浓缩物局限性的替代方案。本研究旨在探讨PEVs对动物模型中培养的真皮乳头细胞（DPCs）和毛囊（HFs）的影响。从新鲜单采血小板中制备并鉴定了PEVs。探讨了它们对DPCs增殖和迁移的影响。在脱毛诱导毛发生长的小鼠模型中皮下注射PEVs后，我们观察到毛囊从休止期到生长期的加速转变，并发现β-连环蛋白信号通路参与其中。这些结果表明，PEVs可有效激活DPCs并促进毛囊生长。单采血小板来源的PEVs为脱发治疗提供了一种有力的替代方法。
