Mai Ruotong, Zhou Yixin, Zhao Kangning, Xie Miao, Tang Yufei, Li Xingrui, Huang Wei, Xiang Lin
State Key Laboratory of Oral Diseases & National Center for Stomatology &, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Department of Oral Implantology, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Mikrochim Acta. 2025 May 5;192(6):335. doi: 10.1007/s00604-025-07189-8.
Intraoral biomarkers are important indicators for the diagnosis and prediction of oral and systemic diseases. Among various intraoral biomarkers, the biomarkers in saliva have been the main focus of research, due to their abundance, non-invasiveness, and correlation with health status. Nonetheless, detecting low-abundance intraoral biomarkers poses significant challenges, and the conventional assays are unsuitable for swift large-scale analysis due to their complex procedures. Hence, an immediate demand arises for innovative methods to supplant traditional assay techniques. Organic transistor-based biosensors have emerged as promising devices for the detection of these intraoral biomarkers, especially in point-of-care (POC) settings. These biosensors offer advantages such as high sensitivity, selectivity, ease of integration, and biocompatibility. This review provides an overview of the evolution and utilization of biosensors that rely on functional organic transistors, with a focus on electrolyte-gated organic field-effect transistors (EGOFETs) and organic electrochemical transistors (OECTs). First, the working principles and sensing mechanisms of various organic transistors are summarized. Then, recent progress and challenges in developing organic transistor-based biosensing platforms for detecting intraoral biomarkers are summarized, along with examples from representative studies. Last, prospects and opportunities for the advancement of organic transistor-based biosensors for oral health monitoring are discussed.
口腔内生物标志物是口腔和全身疾病诊断与预测的重要指标。在各种口腔内生物标志物中，唾液中的生物标志物因其丰富性、非侵入性以及与健康状况的相关性，一直是研究的主要焦点。尽管如此，检测低丰度口腔内生物标志物面临重大挑战，而且传统检测方法由于程序复杂，不适用于快速大规模分析。因此，迫切需要创新方法来取代传统检测技术。基于有机晶体管的生物传感器已成为检测这些口腔内生物标志物的有前景的设备，尤其是在即时检测（POC）环境中。这些生物传感器具有高灵敏度、选择性、易于集成和生物相容性等优点。本综述概述了依赖功能性有机晶体管的生物传感器的发展与应用，重点关注电解质门控有机场效应晶体管（EGOFET）和有机电化学晶体管（OECT）。首先，总结了各种有机晶体管的工作原理和传感机制。然后，总结了开发用于检测口腔内生物标志物的基于有机晶体管的生物传感平台的最新进展和挑战，并列举了代表性研究的实例。最后，讨论了基于有机晶体管的生物传感器在口腔健康监测方面的发展前景和机遇。
