从玫瑰茄废渣中鉴定多酚并评价其体外和体内抗氧化活性。
Identification of polyphenols from Rosa roxburghii Tratt pomace and evaluation of in vitro and in vivo antioxidant activity.
机构信息
SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
SCUT-Zhuhai Institute of Modern Industrial Innovation, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
出版信息
Food Chem. 2022 May 30;377:131922. doi: 10.1016/j.foodchem.2021.131922. Epub 2021 Dec 29.
Rosa roxburghii Tratt pomace (RRTP) has increasingly attracted attention due to its various nutritional ingredients and health benefits. In this study, the free phenolic fraction (RRTP-FPF) and bound phenolic fraction (RRTP-BPF) were extracted from RRTP by solvent extraction method and alkaline hydrolysis method, respectively. The composition of polyphenols in RRTP-FPF and RRTP-BPF were identified by ultra-high performance liquid chromatography equipped with an electrospray ionization and quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-MS/MS). In vitro antioxidant assays indicated that RRTP-FPF and RRTP-BPF could scavenge radicals in a dose-dependent manner, and RRTP-BPF exhibited better scavenging activity than RRTP-FPF. In addition, RRTP-FPF and RRTP-BPF (20 ∼ 100 μg/mL) treatment for 24 h could significantly increase the survival rate and decrease reactive oxygen species (ROS) level of paraquat-exposed nematodes through improving the activities of superoxide dismutase (SOD) and catalase (CAT). These results suggest that RRTP could be as a good and cheap source of natural antioxidants.
罗田甜柿渣（RRTP）因其各种营养成分和健康益处而受到越来越多的关注。本研究采用溶剂萃取法和碱水解法从 RRTP 中提取游离酚部分（RRTP-FPF）和结合酚部分（RRTP-BPF），并采用超高效液相色谱-电喷雾电离-四极杆飞行时间质谱联用仪（UHPLC-ESI-QTOF-MS/MS）鉴定 RRTP-FPF 和 RRTP-BPF 中的多酚组成。体外抗氧化试验表明，RRTP-FPF 和 RRTP-BPF 均可剂量依赖性地清除自由基，RRTP-BPF 的清除活性优于 RRTP-FPF。此外，RRTP-FPF 和 RRTP-BPF（20~100μg/mL）处理 24 h 可通过提高超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，显著提高百草枯暴露线虫的存活率并降低活性氧（ROS）水平。这些结果表明，RRTP 可能是一种良好且廉价的天然抗氧化剂来源。
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