Saito Koichi, Abe Musashi, Ito Rie, Akiyama Hiroshi
Department of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41Shinagawa-ku, Ebara, Tokyo, 142-8501, Japan.
Anal Sci. 2025 May 6. doi: 10.1007/s44211-025-00782-0.
A systematic analytical method for discriminating methamphetamine (MA) from its structural analogs was developed by combining a solid-phase chromogenic method with the use of a molecularly imprinted polymer (MIP) and Simon reaction. L-proline (Pro), L-hydroxyproline (HYP), N-methylbenzylamine (NMe-BA), and N-isopropylbenzylamine (NIP-BA) were selected as false positives, while tert-butoxycarbonyl derivatized MA (t-Boc-MA) was selected as a false-negative substance. MA and the structural analogs were loaded into SupelMIP™ SPE-Amphetamines cartridges and Simon's reagent was added to the cartridge, then the coloration of the solid-phase gel was observed from outside the cartridge. After elution with a basic solution, Simon's reagent was added to the eluate (first-step eluate), and a change in color tone was observed. The MIP was then heated under acidic conditions, and the analytes were eluted (second-step eluate) with water, basified at pH 10, and the Simon reaction was repeated. Pro and HYP could be discriminated from MA by the color tone of the MIP solid-phase gel, whereas NMe-BA and NIP-BA could be discriminated from MA by the color tone of the first-step eluate. In addition, the color tone of the second-step eluate confirmed the presence of t-Boc-MA.
通过将固相显色法与分子印迹聚合物（MIP）的使用及西蒙反应相结合，开发了一种用于区分甲基苯丙胺（MA）与其结构类似物的系统分析方法。选择L-脯氨酸（Pro）、L-羟脯氨酸（HYP）、N-甲基苄胺（NMe-BA）和N-异丙基苄胺（NIP-BA）作为假阳性物质，而叔丁氧羰基衍生化的MA（t-Boc-MA）被选为假阴性物质。将MA及其结构类似物加载到SupelMIP™ SPE-安非他明柱中，向西蒙试剂加入到柱中，然后从柱外部观察固相凝胶的显色情况。用碱性溶液洗脱后，向西蒙试剂加入到洗脱液（第一步洗脱液）中，并观察色调变化。然后在酸性条件下加热MIP，用水洗脱分析物（第二步洗脱液），将pH调至10进行碱化，并重复西蒙反应。通过MIP固相凝胶的色调可以将Pro和HYP与MA区分开来，而通过第一步洗脱液的色调可以将NMe-BA和NIP-BA与MA区分开来。此外，第二步洗脱液的色调证实了t-Boc-MA的存在。
