Deng Yanmei, Lu Yanling, Jiang Yuxin, Yuan Guohui, Yang Tongqin, Gao Baoyu, Yang Jiaojiao, Guo Lei, Fan Fangyu
College of Biological and Food Engineering, Southwest Forestry University, Kunming 650224, China.
College of Biological and Food Engineering, Southwest Forestry University, Kunming 650224, China; Key Laboratory of Forest Disaster Warning and Control of Yunnan Province, Kunming 650224, China; Key Laboratory of National Forestry and Grassland Administration on Biodiversity Conservation in Southwest China, Southwest Forestry University, Kunming 650224, China.
Int J Biol Macromol. 2025 Jun;311(Pt 2):143693. doi: 10.1016/j.ijbiomac.2025.143693. Epub 2025 May 4.
Cold plasma (CP) was used to modify walnut protein isolate (WalPI), and the effects of different CP treatment times (30 s, 60 s, 90 s, 120 s, and 150 s) on structural and functional properties, along with the modification mechanism, were investigated. The results showed that CP treatment altered the structure of WalPI, primarily by unfolding the tertiary structure and modifying the secondary structure. These modifications included an increase in fluorescence intensity, a decrease in α-helix content, and an increase in β-sheet and random coil content. The three-dimensional network structure exhibited crystalline characteristics, and the microstructure displayed irregular spherical particles. These structural changes led to an improvement in the functional properties, as evidenced by a reduction in average particle size, an increase in solubility to 11.42 %, and improvements in emulsification activity index and emulsification stability index, which increased from 0.50 m/g and 1.22 min to 2.75 m/g and 30.50 min. Additionally, water and oil holding capacity increased from 0.45 g/g and 2.54 g/g to 1.21 g/g and 7.46 g/g. Notably, the optimal comprehensive properties of WalPI were achieved after 90 s of CP treatment. This study provides a theoretical foundation for modifying WalPI and enhances its potential for resource utilization.
采用冷等离子体（CP）对核桃分离蛋白（WalPI）进行改性，研究了不同CP处理时间（30 s、60 s、90 s、120 s和150 s）对其结构和功能特性的影响以及改性机理。结果表明，CP处理改变了WalPI的结构，主要是通过展开三级结构和修饰二级结构来实现的。这些修饰包括荧光强度增加、α-螺旋含量降低以及β-折叠和无规卷曲含量增加。三维网络结构呈现出晶体特征，微观结构显示出不规则的球形颗粒。这些结构变化导致功能特性得到改善，表现为平均粒径减小、溶解度提高到11.42%，乳化活性指数和乳化稳定性指数也得到改善，分别从0.50 m/g和1.22 min增加到2.75 m/g和30.50 min。此外，持水能力和持油能力分别从0.45 g/g和2.54 g/g增加到1.21 g/g和7.46 g/g。值得注意的是，CP处理90 s后WalPI的综合性能达到最佳。本研究为WalPI的改性提供了理论基础，提高了其资源利用潜力。
