炎症性肠病中RNF186基因变异与连蛋白水平：一项初步研究。
RNF186 gene variant and zonulin levels in inflammatory bowel disease: A pilot study.
作者信息
Güneri Elif Özdemir, Kahraman Resul, Ataş Merve Nur, Ergen Arzu
机构信息
Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkiye; Institute of Graduate Studies in Health Sciences, Istanbul University, Istanbul, Turkiye.
Department of Gastroenterology, Umraniye Education and Research Hospital, Istanbul, Turkiye.
出版信息
Arab J Gastroenterol. 2025 May;26(2):207-211. doi: 10.1016/j.ajg.2025.02.005. Epub 2025 May 5.
BACKGROUND AND STUDY AIM
Inflammatory bowel disease (IBD) is a chronic inflammatory disorder of the gastrointestinal tract characterized by inflammation and ulceration of the intestinal mucosa, represented by Crohn's disease (CD) and ulcerative colitis (UC). The aim of this study is to investigate the relationship between the Ring Finger Protein 186 (RNF186) rs3806308 variant and IBD, as well as to determine whether zonulin levels are associated with IBD.
PATIENTS AND METHODS
Ninety-nine patients with inflammatory bowel disease were included in the study. The real-time PCR method was used to detect RNF186 gene polymorphism. Also serum zonulin levels were determined by using Enzyme Linked Immunosorbent Assay (ELISA) technique.
RESULTS
No difference was found between the groups in terms of RNF186 genotype and allele distributions (p > 0.05). CC genotype was associated with high levels of C-reactive protein (CRP) in total patients and CD compared to CT (p < 0.05).
CONCLUSION
The present study is the first study conducted in our country in terms of examining RNF 186 gene polymorphism and serum zonulin levels.
背景与研究目的
炎症性肠病（IBD）是一种胃肠道慢性炎症性疾病，其特征为肠道黏膜炎症和溃疡，以克罗恩病（CD）和溃疡性结肠炎（UC）为代表。本研究旨在探讨环指蛋白186（RNF186）rs3806308变异与IBD之间的关系，并确定zonulin水平是否与IBD相关。
患者与方法
本研究纳入了99例炎症性肠病患者。采用实时聚合酶链反应（PCR）方法检测RNF186基因多态性。同时，使用酶联免疫吸附测定（ELISA）技术测定血清zonulin水平。
结果
RNF186基因型和等位基因分布在各组之间无差异（p>0.05）。与CT基因型相比，CC基因型在所有患者和CD患者中与高水平的C反应蛋白（CRP）相关（p<0.05）。
结论
本研究是我国首次进行的关于检测RNF186基因多态性和血清zonulin水平的研究。
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