Cenni Sabrina, Casertano Marianna, Trani Marco, Pacella Daniela, Martinelli Massimo, Staiano Annamaria, Miele Erasmo, Strisciuglio Caterina
Department of Experimental Medicine, University of Campania "Luigi Vanvitelli", Naples, Italy.
Department of Woman, Child and General and Specialist Surgery, University of Campania "Luigi Vanvitelli", Naples, Italy.
Eur J Pediatr. 2023 Mar;182(3):1299-1308. doi: 10.1007/s00431-022-04771-7. Epub 2023 Jan 13.
Calgranulin-C (S100A12) and zonulin are considered markers of intestinal inflammation. Our aim was to evaluate fecal S100A12 (f-S100A12) and fecal zonulin (f-zonulin) in children with inflammatory bowel disease (IBD), compared to fecal calprotectin (FC) and serum inflammatory markers. We enrolled children with a previous diagnosis of Crohn's disease (CD) and ulcerative colitis (UC). F-S100A12, f-zonulin, and FC were determined by enzyme-linked immunosorbent assay (ELISA). Endoscopic examination was considered in the patients who underwent ileocolonoscopy within 2 weeks from the enrollment. One hundred seventeen children, 39.3% with CD and 60.7% with UC were enrolled. In both CD and UC, there was a significant direct correlation between FC and f-S100A12 levels. In children with CD and UC, both FC and f-S100A12 correlated with markers of serum inflammation. We found difference in FC and f-S100A12 levels between patients in clinical relapse and remission (FC: mean 1027 ± 818 mcg/ml vs 580 ± 695 mcg/ml respectively, p = 0.028; f-S100A12: mean 66.4 ± 48.2 mcg/ml vs 42.7 ± 40 mcg/ml, respectively p = 0.02). Moreover, we found difference in FC between children with endoscopic inflammation and remission (mean 825 ± 779 mcg/ml vs 473.3 ± 492 mcg/ml, respectively p = 0.048), as well as for f-S100A12 (53 ± 43 mcg/ml vs mean 31 ± 33 mcg/ml vs, respectively p = 0.019). No significant results were found for f-zonulin.
Our data suggest that f-S100A12 and FC are both useful non-invasive biomarkers in the management of pediatric IBD in follow up and in monitoring endoscopic and clinical relapse.
• Fecal calprotectin (FC), fecal S100A12 (f- S100A12), and fecal zonulin represent potential noninvasive markers of gut inflammation. • Since S100A12 is predominantly expressed by granulocytes, high levels of f-S100A12 should be more specific for inflammation than FC.
• FC and f-S100A12 were correlated to each other and despite the lack of correlation with disease location, they were associated with endoscopic inflammation and clinical relapse in children with IBD. • No significant correlations were found between f-zonulin and the inflammatory parameters.
钙粒蛋白-C（S100A12）和zonulin被视为肠道炎症的标志物。我们的目的是评估炎症性肠病（IBD）患儿的粪便S100A12（f-S100A12）和粪便zonulin（f-zonulin），并与粪便钙卫蛋白（FC）和血清炎症标志物进行比较。我们纳入了先前诊断为克罗恩病（CD）和溃疡性结肠炎（UC）的患儿。通过酶联免疫吸附测定（ELISA）测定f-S100A12、f-zonulin和FC。在入组后2周内接受回结肠镜检查的患者进行了内镜检查。共纳入117名患儿，其中39.3%患有CD，60.7%患有UC。在CD和UC中，FC与f-S100A12水平之间均存在显著的正相关。在患有CD和UC的患儿中，FC和f-S100A12均与血清炎症标志物相关。我们发现临床复发和缓解患者的FC和f-S100A12水平存在差异（FC：分别为平均1027±818 mcg/ml和580±695 mcg/ml，p = 0.028；f-S100A12：分别为平均66.4±48.2 mcg/ml和42.7±40 mcg/ml，p = 0.02）。此外，我们发现内镜炎症和缓解患儿的FC存在差异（分别为平均825±779 mcg/ml和473.3±492 mcg/ml，p = 0.048），f-S100A12也存在差异（53±43 mcg/ml和平均31±33 mcg/ml，p = 0.019）。f-zonulin未发现显著结果。
我们的数据表明，f-S100A12和FC都是儿科IBD随访以及监测内镜和临床复发管理中有用的非侵入性生物标志物。
• 粪便钙卫蛋白（FC）、粪便S100A12（f-S100A12）和粪便zonulin是肠道炎症的潜在非侵入性标志物。• 由于S100A12主要由粒细胞表达，高水平的f-S100A12对炎症的特异性应高于FC。
• FC和f-S100A12相互相关，尽管与疾病部位缺乏相关性，但它们与IBD患儿的内镜炎症和临床复发相关。• f-zonulin与炎症参数之间未发现显著相关性。
