在中国发现的一种与诸葛菜相关的新型变叶病毒。

A novel varicosavirus associated with Orychophragmus violaceus in China.

作者信息

Chen Yuanling, Li Qian, Yu Jiaping, Zhou Yali, Fei Shifang, Wu Jianxiang, Fu Shuai

机构信息

Hainan Institute, Zhejiang University, Sanya, 572025, China.

State Key Laboratory of Rice Biology and Breeding, Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province, Institute of Biotechnology, Zhejiang University, Hangzhou, 310058, China.

出版信息

Arch Virol. 2025 May 7;170(6):121. doi: 10.1007/s00705-025-06283-9.

DOI:10.1007/s00705-025-06283-9

PMID:40329141

Abstract

Plant rhabdoviruses have garnered considerable attention due to their broad host range and significant agricultural threats. Despite their prevalence in various ecosystems, rhabdoviruses in non-crop species, which often serve as reservoirs for novel viral strains, remain understudied. Here, a novel plant rhabdovirus, Orychophragmus violaceus varicosavirus (OVVV), was identified in the ornamental plant Orychophragmus violaceus and characterized by high-through sequencing. OVVV has a bisegmented genome, with RNA1 consisting of 6,795 nucleotides (nt) and RNA2 consisting of 5,895 nt. The L protein encoded by RNA1 shares 36.4-67.2% amino acid sequence identity with those of known varicosaviruses. The four proteins encoded by RNA2 share 19.5-53.4% amino acid identity with the corresponding proteins of known varicosaviruses. Sequence comparisons and phylogenetic analysis indicated that OVVV is a novel RNA virus representing a new species within the genus Varicosavirus. This discovery not only expands the known host range of varicosaviruses but also offers new insights into the molecular evolution of this group of viruses.

摘要

植物弹状病毒因其广泛的宿主范围和对农业的重大威胁而备受关注。尽管它们在各种生态系统中普遍存在，但非作物物种中的弹状病毒（这些物种通常是新型病毒株的储存库）仍未得到充分研究。在此，在观赏植物二月兰中鉴定出一种新型植物弹状病毒，即二月兰静脉曲张病毒（OVVV），并通过高通量测序对其进行了表征。OVVV具有双分体基因组，RNA1由6795个核苷酸（nt）组成，RNA2由5895个nt组成。RNA1编码的L蛋白与已知静脉曲张病毒的L蛋白氨基酸序列同一性为36.4%至67.2%。RNA2编码的四种蛋白与已知静脉曲张病毒的相应蛋白氨基酸同一性为19.5%至53.4%。序列比较和系统发育分析表明，OVVV是一种新型RNA病毒，代表静脉曲张病毒属内的一个新物种。这一发现不仅扩大了已知静脉曲张病毒的宿主范围，还为这组病毒的分子进化提供了新的见解。