Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, Liaoning, 110044, China.
National Citrus Engineering and Technology Research Center, Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Citrus Research Institute, Southwest University, Tiansheng Road, Beibei, Chongqing, 400712, China.
Arch Virol. 2024 Oct 4;169(10):212. doi: 10.1007/s00705-024-06141-0.
A putative novel virus was identified in Agastache rugosa in China and tentatively named "Agastache rugosa-associated varicosavirus" (ARaVV). The nearly complete genome sequence of ARaVV was obtained through RNA sequencing (RNA-seq) and subsequent RT-PCR. The ARaVV genome consists of two negative-sense single-stranded RNA segments that are 6428 and 3862 nucleotides (nt) in size, respectively. RNA1 encodes a large polymerase (L), and RNA2 encodes a putative nucleocapsid protein (N), protein 2 (P2), and protein 3 (P3). The L protein shared the highest amino acid (aa) sequence identity (51.3%) with Erysimum virus 1 (EryV1, BK061766.1). The N, P2, and P3 shared the highest aa sequence identity (33.1%, 14.0%, and 24.2%) with Leucanthemum virus 1, Raphanus virus 1, and Spinach virus 1, respectively. Phylogenetic analysis based on amino acid sequences of the L protein showed that ARaVV clustered in a clade with the varicosaviruses, indicating that ARaVV is a putative new member of the genus Varicosavirus.
在中国藿香中发现了一种假定的新型病毒，并暂定名为“藿香相关静脉曲张病毒”（ARaVV）。通过 RNA 测序（RNA-seq）和随后的 RT-PCR 获得了 ARaVV 的几乎完整的基因组序列。ARaVV 基因组由两个负义单链 RNA 片段组成，大小分别为 6428 和 3862 个核苷酸（nt）。RNA1 编码一个大型聚合酶（L），RNA2 编码一个假定的核衣壳蛋白（N）、蛋白 2（P2）和蛋白 3（P3）。L 蛋白与野芥子病毒 1（EryV1，BK061766.1）的氨基酸（aa）序列同源性最高（51.3%）。N、P2 和 P3 与 Leucanthemum virus 1、Raphanus virus 1 和 Spinach virus 1 的 aa 序列同源性最高（33.1%、14.0%和 24.2%）。基于 L 蛋白氨基酸序列的系统发育分析表明，ARaVV 与静脉曲张病毒聚类在一个分支中，表明 ARaVV 是静脉曲张病毒属的一个假定新成员。
