一种从猫杯状病毒（FCV）共感染标本中选择性分离猫疱疹病毒1型（FHV-1）的有效方法。
An efficient method for the selective isolation of feline herpesvirus 1(FHV-1) in feline calicivirus (FCV) coinfected specimens.
作者信息
Zheng Huanqin, Yue Hong, Wang Baoyan, Yu Xin, Liu Yang, Yu Jiayu, Zhang Jianlong, Han Kexue, Han Yinuo, Su Hanfeng, Zhu Hongwei, Zhang Xingxiao
机构信息
School of Life Sciences, Ludong University, No. 186 Hongqi Middle Rd., Zhifu District, Yantai, 264025, China.
Collaborative Innovation Center for the Pet Infectious Diseases and Public Health in the Middle and Lower Stream Regions of the Yellow River, Yantai, 264025, Shandong, China.
出版信息
BMC Vet Res. 2025 May 7;21(1):321. doi: 10.1186/s12917-025-04786-w.
BACKGROUND
Feline herpesvirus 1 (FHV-1) and feline calicivirus (FCV) are the most common viral pathogens of feline respiratory disease and are highly prevalent in cats worldwide. Coinfection with these viruses is frequent in cats with feline respiratory disease complex (FRDC). It is difficult to isolate pure FHV-1 by conventional laboratory cell culture methods from specimens with FRDC, which brings great trouble to the epidemiological investigation of FHV-1.
METHODS
FCV polyclonal antibodies were obtained by immunizing rabbits, and the coinfected specimens were neutralized with FCV polyclonal antibodies. Then, virus isolation was performed. After several rounds of neutralization, FHV-1 was finally obtained.
RESULTS
The FCV polyclonal antibody was successfully obtained with neutralizing activities of 1:128, 1:537, and 1:91. After virus neutralization, the FHV-1 was successfully isolated from the coinfected cell culture suspension and confirmed by immunofluorescence and QRT-PCR.
CONCLUSION
In this study, all FHV-1 present in the coinfection samples were isolated, without any cross-contamination. This method is also theoretically suitable for the isolation and purification of other FCV coinfections or contaminating disease substances.
背景
猫疱疹病毒1型（FHV-1）和猫杯状病毒（FCV）是猫呼吸道疾病最常见的病毒病原体，在全球范围内的猫中高度流行。在患有猫呼吸道疾病综合征（FRDC）的猫中，这些病毒的合并感染很常见。用传统的实验室细胞培养方法很难从患有FRDC的标本中分离出纯FHV-1，这给FHV-1的流行病学调查带来了很大麻烦。
方法
通过免疫兔子获得FCV多克隆抗体，并用FCV多克隆抗体中和合并感染的标本。然后进行病毒分离。经过几轮中和后，最终获得FHV-1。
结果
成功获得了FCV多克隆抗体，其中和活性分别为1:128、1:537和1:91。病毒中和后，从合并感染的细胞培养悬液中成功分离出FHV-1，并通过免疫荧光和定量逆转录聚合酶链反应（QRT-PCR）进行了确认。
结论
在本研究中，合并感染样本中存在的所有FHV-1均被分离出来，没有任何交叉污染。该方法在理论上也适用于其他FCV合并感染或污染性疾病物质的分离和纯化。
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