Yamada Ai, Ishii Kenta, Takeuchi Akito, Tashiro Tomiko, Endo Ginji, Ono-Ogasawara Mariko
Osaka Occupational Health Service Center, Japan Industrial Safety and Health Association, 2-3-8 Tosabori, Nishi-Ku, Osaka 550-0001, Japan.
Department of Pathophysiological Laboratory Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daikominami, Higashi-ku, Nagoya, Aichi 461-8673, Japan.
J Occup Health. 2025 Jan 7;67(1). doi: 10.1093/joccuh/uiaf025.
This study aimed to establish a method to determine workers' exposure concentrations to airborne nitrilotriacetic acid (NTA).
The sampler used an air sampling cassette containing a glass-fiber filter (GFF). After sampling, NTA extracted from the GFF using ultrapure water was derivatized using a boron trifluoride-methanol complex methanol solution and analyzed using a gas chromatograph-mass spectrometer. The developed method was validated for the following parameters: retention efficiency, storage stability, method quantitation limit, and reproducibility.
The retention efficiency for NTA on the GFF was 94%-101%, with the relative SD indicating the overall reproducibility (range 0.9%-2.4%). NTA on the GFF was stable at 4°C for at least 7 days. The method quantitation limit was 4.8 μg per sample.
The developed method will be useful for risk assessments because it can determine workers' exposure concentrations to NTA ranging from 0.02 to 4 mg/m3 in a 240 L sampling volume; the quantitation limit is 4.8 μg per sample.
本研究旨在建立一种测定工人空气中次氮基三乙酸（NTA）暴露浓度的方法。
采样器使用装有玻璃纤维滤膜（GFF）的空气采样盒。采样后，用超纯水从GFF中提取的NTA用三氟化硼 - 甲醇络合物甲醇溶液进行衍生化，并使用气相色谱 - 质谱仪进行分析。所开发的方法针对以下参数进行了验证：保留效率、储存稳定性、方法定量限和重现性。
NTA在GFF上的保留效率为94% - 101%，相对标准偏差表明总体重现性（范围为0.9% - 2.4%）。GFF上的NTA在4°C下至少稳定7天。方法定量限为每个样品4.8μg。
所开发的方法对于风险评估将是有用的，因为它可以在240L采样体积中测定工人对NTA的暴露浓度，范围为0.02至4mg/m³；定量限为每个样品4.8μg。
