Zhu Wanjun, Shao Ping, Ren Ying, Liu Wenxuan, Han Xiaorui, Zeng Kexin, Dai Chunmei, Liu Feifei
School of Basic Medicine, Jinzhou Medical University, Jinzhou, Liaoning, China.
Benxi National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines Co., Ltd., Benxi, Liaoning, China.
Front Cardiovasc Med. 2025 Apr 22;12:1512791. doi: 10.3389/fcvm.2025.1512791. eCollection 2025.
Gualou Xiebai Banxia Decoction (GXBD), a traditional Chinese medicine, is used to treat myocardial ischemia (MI). However, the molecular mechanisms underlying its effects remain unclear. This study integrated network pharmacology and experimental validation to investigate the efficacy and potential mechanisms of GXBD in MI treatment.
Network pharmacology was used to predict the mechanism of action of GXBD in MI. The predicted results were verified using ECG, echocardiography, HE staining, TTC staining, DHE, JC-1, immunofluorescence, and Western blot analysis in an isoproterenol (ISO)-induced MI rat model.
Network pharmacology identified 33 active components in GXBD and 139 potential targets against MI, with the PI3K/AKT signaling pathway playing a key role. Compared to the model group, GXBD improved the activities of BNP, CK-MB, and LDH, ameliorated the general condition and cardiac function, and repaired heart damage in MI rats. GXBD decreased MDA and ROS levels, increased SOD and GSH-Px levels, and protected cardiac tissues from oxidative stress. Moreover, GXBD increased ATP content, mitochondrial membrane potential, and the levels of p-PI3K, p-AKT, nuclear NRF2, and MFN2, while decreasing the levels of cytoplasmic NRF2 and DRP1.
This study suggested that GXBD alleviates myocardial ischemia by ameliorating mitochondrial dysfunction through the PI3K/AKT/NRF2 signaling pathway.
瓜蒌薤白半夏汤（GXBD）是一种传统中药，用于治疗心肌缺血（MI）。然而，其作用的分子机制尚不清楚。本研究综合网络药理学和实验验证，以探讨GXBD治疗MI的疗效和潜在机制。
采用网络药理学预测GXBD在MI中的作用机制。在异丙肾上腺素（ISO）诱导的MI大鼠模型中，通过心电图、超声心动图、HE染色、TTC染色、DHE、JC-1、免疫荧光和蛋白质印迹分析对预测结果进行验证。
网络药理学鉴定出GXBD中的33种活性成分和139个抗MI潜在靶点，PI3K/AKT信号通路起关键作用。与模型组相比，GXBD改善了MI大鼠的BNP、CK-MB和LDH活性，改善了一般状况和心脏功能，并修复了心脏损伤。GXBD降低了MDA和ROS水平，提高了SOD和GSH-Px水平，保护心脏组织免受氧化应激。此外，GXBD增加了ATP含量、线粒体膜电位以及p-PI3K、p-AKT、核NRF2和MFN2的水平，同时降低了细胞质NRF2和DRP1的水平。
本研究表明，GXBD通过PI3K/AKT/NRF2信号通路改善线粒体功能障碍，从而减轻心肌缺血。
