Israyelyan Arevik, Balabekyan Tsovinar, Aleksanyan Lara, Sahakyan Inesa, Gasparyan Anna, Tkhruni Flora
Scientific and Production Center "Armbiotechnology" NSPO, National Academy of Science, Probiotics Production Sector, Yerevan, Republic of Armenia.
Department Histochemistry and Functional Morphology, Buniatyan Institute of Biochemistry National Academy of Science, Yerevan, Republic of Armenia.
Iran J Microbiol. 2025 Feb;17(1):113-121. doi: 10.18502/ijm.v17i1.17808.
It has been shown that strains of the genus isolated from donkey milk from different regions of the Republic of Armenia have antimicrobial activity, synthesize different polysaccharides and produce disaccharide polymers (glucose and galactose). The quantitative synthesis of polysaccharides (8-15%) depends on the composition of the nutrient medium, temperature and growing time.
Species identification of LAB strains was confirmed by 16S rDNA gene sequencing method using universal primers LAB strains. The methods used for extraction, purification and detection of exopolysaccharides are based on the method of Sørensen et al. (2022). The antibacterial activity of EPS was investigated by agar diffusion assay. Determination of the immunostimulating property was carried out using the ELISA method.
The antimicrobial activity of the polysaccharide and antimicrobial protein-like fractions of the genus strains depends on its concentration, time of interaction with the test culture, and the species of the pathogenic bacteria.
The obtained results were shown that strains isolated from fermented donkey milk that are capable of synthesizing two substances of different nature with high antimicrobial properties during the growth process are promising for further research and application for their use as probiotics and biopreparations in pharmaceuticals.
已表明从亚美尼亚共和国不同地区的驴奶中分离出的该属菌株具有抗菌活性，能合成不同的多糖并产生二糖聚合物（葡萄糖和半乳糖）。多糖的定量合成（8 - 15%）取决于营养培养基的组成、温度和生长时间。
使用通用引物通过16S rDNA基因测序法对乳酸菌菌株进行种属鉴定。用于胞外多糖提取、纯化和检测的方法基于索伦森等人（2022年）的方法。通过琼脂扩散法研究胞外多糖的抗菌活性。使用酶联免疫吸附测定法测定免疫刺激特性。
该属菌株的多糖和类抗菌蛋白组分的抗菌活性取决于其浓度、与测试培养物的相互作用时间以及病原菌的种类。
所得结果表明，从发酵驴奶中分离出的菌株在生长过程中能够合成两种具有高抗菌特性的不同性质的物质，有望进一步研究并应用于作为药物中的益生菌和生物制剂。
