Geng Rui, Fan Xiang, Sarwar Rehman, Wang Yong, Dong Ke, Tan Xiao-Li
School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China.
School of Life Sciences, Jiangsu University, Zhenjiang, China.
Front Plant Sci. 2025 Apr 22;16:1572020. doi: 10.3389/fpls.2025.1572020. eCollection 2025.
Traditional rapeseed breeding is inefficient and imprecise. CRISPR genome editing offers a precise alternative for trait improvement. Here, we edited the gene in elite rapeseed cultivar ZS11 to study its role in floral organ abcission and enable rapid trait transfer to elite lines.
The gene was CRISPR-edited in ZS11. Phenotypes (petal adhesion time, cracking force of siliques) were statistically analyzed. And analyze the mutants using RNA -Seq. Edited alleles were introgressed into elite line SW1-6 via backcrossing. Locus-specific primers enabled efficient genotyping to distinguish hetero- and homozygous plants during selection.
In this study, The mutant by gene editing in the cv ZS11, which is widely used in rapeseed breeding. The phenotypic analysis showed that the petal was attached to the pod and pods were harder to crack in edited plants, and then we quickly introduced two loci into the elite line of SW1-6 by backcrossing with edited ZS11 as the donor plant. Locus-specific primer combinations were designed to differentiate heterozygous and homozygous genotypes in backcrossing generations, enabling efficient and rapid selection. This study highlights the integration of gene editing and genotyping selection, offering insights into the future of gene editing-assisted breeding.
传统油菜育种效率低下且不准确。CRISPR基因组编辑为性状改良提供了一种精确的替代方法。在此，我们对优良油菜品种中双11（ZS11）中的 基因进行编辑，以研究其在花器官脱落中的作用，并实现向优良品系的快速性状转移。
在中双11（ZS11）中对 基因进行CRISPR编辑。对表型（花瓣附着时间、角果开裂力）进行统计分析。并使用RNA-Seq分析突变体。通过回交将编辑后的等位基因导入优良品系SW1-6。位点特异性引物能够在选择过程中进行高效基因分型，以区分杂合和纯合植株。
在本研究中，通过对广泛用于油菜育种的中双11（cv ZS11）进行基因编辑获得了 突变体。表型分析表明，在编辑后的植株中，花瓣附着在角果上，角果更难开裂，然后我们以编辑后的中双11为供体植株，通过回交将两个 位点快速导入优良品系SW1-6。设计了位点特异性引物组合，以区分回交后代中的杂合和纯合基因型，从而实现高效快速的选择。本研究突出了基因编辑与基因分型选择的整合，为基因编辑辅助育种的未来发展提供了见解。
