利用改良的CRISPR/Cas9系统在甘蓝中创制具有广谱抗性的新型bpm6和dmr6突变体。

Generation of novel bpm6 and dmr6 mutants with broad-spectrum resistance using a modified CRISPR/Cas9 system in Brassica oleracea.

作者信息

Zhang Yulun, Liu Jinhui, Li Yingjie, Ma Hongxue, Ji Jialei, Wang Yong, Zhuang Mu, Yang Limei, Fang Zhiyuan, Li Jun, Zhang Chao, Liu Liwang, Lebedeva Marina, Taranov Vasiliy, Zhang Yangyong, Lv Honghao

机构信息

State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 100096, China.

出版信息

J Integr Plant Biol. 2025 May;67(5):1214-1216. doi: 10.1111/jipb.13842. Epub 2025 Jan 28.

DOI:10.1111/jipb.13842

PMID:39873367

Abstract

Using an optimized CRISPR/Cas9 system to knock out the BTB-POZ and MATH domain gene BoBPM6 and the DOWNY MILDEW RESISTANCE 6 gene in Brassica oleracea resulted in new lines with broad-spectrum disease resistance.

摘要

利用优化的CRISPR/Cas9系统敲除甘蓝中的BTB-POZ和MATH结构域基因BoBPM6以及抗霜霉病6基因，获得了具有广谱抗病性的新品系。