Cevallos Carlos A, White Anna Leigh, Fazio Brooke A, Wendt Lillian S, Feng Jasmine W, Posfai Dora, Horton April L, Warrick John M, Quintero-Carmona Omar Alberto
Department of Biology, University of Richmond, Richmond, Virginia, United States.
Department of Molecular Genetics and Microbiology, Duke University, Durham, North Carolina, United States.
MicroPubl Biol. 2025 Apr 21;2025. doi: 10.17912/micropub.biology.001574. eCollection 2025.
CAD cells were derived from Cath.a cells, a mouse central nervous system catecholaminergic cell line. Serum-starved CAD cells undergo morphological changes and resemble isolated neurons when observed by microscopy. We carried out an RNAseq transcriptomic analysis to examine differentiated CAD cells for expression signatures related to neuronal functions, identifying ~1900 transcripts whose expression changed with differentiation. Pathview analysis identified ~80 KEGG pathway gene sets that were differentially expressed, including upregulation of at least 13 neuron-related pathways. This dataset can be explored more deeply, allowing further investigation into expression changes relevant to studying neuronal functions in this easy-to-culture model system.
CAD细胞源自Cath.a细胞，一种小鼠中枢神经系统儿茶酚胺能细胞系。血清饥饿的CAD细胞会发生形态变化，在显微镜下观察时类似于分离的神经元。我们进行了RNAseq转录组分析，以检查分化的CAD细胞中与神经元功能相关的表达特征，鉴定出约1900个随着分化而表达发生变化的转录本。Pathview分析确定了约80个差异表达的KEGG通路基因集，包括至少13条与神经元相关通路的上调。该数据集可以更深入地探索，从而能够在这个易于培养的模型系统中进一步研究与神经元功能相关的表达变化。
