Wang Yaxuan, Wang Xinfeng, Zhang Kunjie, Xiao Jing, Liu Fang, Yang Houhong, Cai Yubiao, Lai Fengxiang, Fu Qiang, Wan Pinjun
The National Key Laboratory of Rice Biological Breeding, China National Rice Research Institute, Hangzhou 311401, China.
Int J Mol Sci. 2025 Apr 8;26(8):3486. doi: 10.3390/ijms26083486.
The brown planthopper (BPH) is one of the major rice pests causing significant damage to rice production worldwide, due to its high reproductive capacity and strong migratory ability. A series of BPH-resistant rice varieties have been developed, but the durability of effective resistance is constrained by the evolution of BPH virulence, requiring in-depth insights into resistance mechanisms. In this paper, we used strand-specific lncRNA-seq to characterize the lncRNA regulatory mechanisms on rice response to BPH infestation. Overall, 4321 lncRNAs were identified, 60 of which were significantly upregulated in response to BPH infestation, specifically differing between BPH populations with variable virulence. Differential expression analysis and qRT-PCR validation showed that these lncRNAs are involved in the regulation of several defense pathways, including jasmonic acid signaling and flavonoid biosynthesis, with their distinct roles in resistant and susceptible rice varieties. Notably, lncRNAs like were found to be negatively correlated with flavonoid biosynthesis, suggesting a potential role in modulating rice defense responses. In contrast, and were positively correlated with genes involved in glutathione metabolism, which may be associated with enhanced resistance. These findings highlight the critical regulatory functions of lncRNAs in rice-BPH interactions and provide a molecular framework for improving rice resistance through targeted genetic engineering. This study significantly contributes to functional genomics by elucidating lncRNA-mediated regulatory mechanisms and offers promising avenues for developing durable pest-resistant rice varieties.
褐飞虱是对全球水稻生产造成重大损害的主要水稻害虫之一，因其具有高繁殖能力和强迁飞能力。一系列抗褐飞虱的水稻品种已被培育出来，但有效抗性的持久性受到褐飞虱毒力进化的限制，这需要深入了解抗性机制。在本文中，我们使用链特异性lncRNA测序来表征lncRNA对水稻响应褐飞虱侵染的调控机制。总体而言，共鉴定出4321个lncRNA，其中60个在响应褐飞虱侵染时显著上调，在具有不同毒力的褐飞虱种群之间存在特异性差异。差异表达分析和qRT-PCR验证表明，这些lncRNA参与了多种防御途径的调控，包括茉莉酸信号传导和类黄酮生物合成，它们在抗性和感病水稻品种中发挥着不同的作用。值得注意的是，发现如 这样的lncRNA与类黄酮生物合成呈负相关，表明其在调节水稻防御反应中具有潜在作用。相反， 和 与参与谷胱甘肽代谢的基因呈正相关，这可能与增强抗性有关。这些发现突出了lncRNA在水稻-褐飞虱相互作用中的关键调控功能，并为通过靶向基因工程提高水稻抗性提供了分子框架。本研究通过阐明lncRNA介导的调控机制，为功能基因组学做出了重要贡献，并为培育持久抗虫水稻品种提供了有前景的途径。
