Ye Xing, Hu Xingqiang, Zhen Kun, Meng Jing, Du Heyan, Cao Xueye, Zhou Dangwei
The College of Pharmacy, Qinghai Minzu University, Xining 810007, China.
Key Laboratory for Tibet Plateau Phytochemistry of Qinghai Province, Xining 810007, China.
Int J Mol Sci. 2025 Apr 11;26(8):3593. doi: 10.3390/ijms26083593.
-methyladenosine (mA) RNA modification plays important regulatory roles in plant development and adaptation to the environment. However, there has been no research regarding mA RNA methyltransferases (MT-A70) in Maxim. Here, we performed a comprehensive analysis of the MT-A70 family in (), including gene structures, phylogenetic relationships, conserved motifs, gene location, promoter analysis, GO enrichment analysis, and expression profiles. We identified seven genes. Phylogeny analysis indicated that the seven genes could be divided into three groups; two MTA genes, three MTB genes, and two MTC genes, and domains and motifs exhibited similar patterns within the same group. These genes were found to contain a large number of cis-acting elements associated with plant hormones, light response, and stress response, suggesting their widespread regulatory function. Furthermore, the expression profiling of different tissues was investigated using RNA-seq data, and the expression of seven genes was further validated by qPCR analysis. These results provided valuable information to further elucidate the function of mA regulatory genes and their epigenetic regulatory mechanisms in .
N6-甲基腺苷（mA）RNA修饰在植物发育和环境适应中发挥着重要的调控作用。然而，关于紫花苜蓿中mA RNA甲基转移酶（MT-A70）尚未有研究。在此，我们对紫花苜蓿中的MT-A70家族进行了全面分析，包括基因结构、系统发育关系、保守基序、基因定位、启动子分析、GO富集分析和表达谱分析。我们鉴定出了7个MT-A70基因。系统发育分析表明，这7个MT-A70基因可分为三组：两个MTA基因、三个MTB基因和两个MTC基因，同一组内的结构域和基序呈现出相似的模式。发现这些MT-A70基因含有大量与植物激素、光响应和胁迫响应相关的顺式作用元件，表明它们具有广泛的调控功能。此外，利用RNA-seq数据研究了不同组织的表达谱，并通过qPCR分析进一步验证了7个基因的表达。这些结果为进一步阐明紫花苜蓿中mA调控基因的功能及其表观遗传调控机制提供了有价值的信息。
